[Histonet] Separation Artifact

[Tony Henwood]

Dear thecitan <@t> yahoo.com (whoever this is)

I would suggest that to fix the problem, make sure you fix the tissues.

Shrinkage is often due to alcohol "fixation" rather than formal fixation

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
thecitan <@t> yahoo.com
Sent: Tuesday, 22 September 2009 6:27 AM
To: Histonet
Subject: [Histonet] Separation Artifact


Hello histonet!

I'm trying to figure out the cause of some artifact in my ffpe h&e skin
slides. Every once and a while I get a batch of slides with this strange
separation artifact mostly around melanocytes. Its like there is a space
around each cell(s). Also the collagen seems to have some odd
stretching.

I tried recuting to make sure there was no microtomy issues but the
artifact remains. I check my waterbath temp / soaked the blocks. Not
soaked the blocks. I'm at a loss. This happens to entire processing
batch. The doctors say they are still pathologically readable but I
still want to find a way to fix this. Sent from my Verizon Wireless
BlackBerry


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