[Histonet] RE: Histonet Digest, Vol 70, Issue 24
aazath <@t> hotmail.com
aazath <@t> hotmail.com
Sun Sep 20 04:50:38 CDT 2009
Dear All,
I am using Haris's hematoxylin.some times its weak.Is any body have method to get a strong hematoxylin consistently.
Aazath
Department of pathology
Apollo hospitals.
-original message-
Subject: Histonet Digest, Vol 70, Issue 24
From: histonet-request <@t> lists.utsouthwestern.edu
Date: 19/09/2009 10:30 pm
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Today's Topics:
1. Re: Polarizing filters (Robert Richmond)
2. Whole Mount Coverslips (Metzger, Kenneth)
3. RE: Whole Mount Coverslips (Shirley A. Powell)
4. black plastic incubation boxes (Jennifer Anderson)
5. RE: Whole Mount Coverslips (Jack Ratliff)
6. RE: Ultra-Low Temp. Upright Freezer (Hugh Luk)
----------------------------------------------------------------------
Message: 1
Date: Fri, 18 Sep 2009 13:26:42 -0400
From: Robert Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: Polarizing filters
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<abea52a60909181026h2e72f627v599f7e8783f1771d <@t> mail.gmail.com>
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I am really appalled by this thread. In any specialty but pathology, a
physician would not be required to go to a camera store for his
optical equipment, or look through polarizing sun glasses at a
specimen. But it's what's out there - very few pathologists I work for
have polarizers on their microscopes.
A pathologist's microscope should have built-in polarization, and the
system should include a first order plate (full wave plate) for
determining the birefringence direction of crystals (needed for
identifying monosodium urate, a common diagnostic question).
When I was ten years old in 1949 I had a polarizer on my Dad's 1923
medical school monocular brass tube Leitz - I wish I had that scope
today (my sister has it, in a garage in L.A.) (I was an avid rockhound
- still am.) Why can't I have one 60 years later?
I don't mind buying my bone saws from Home Depot and my OptiVISION
magnifier from Amazon, but when it comes to my microscope I'd sort of
like to have the real thing.
Bob Richmond
Samurai Pathologist
Knoxville TN
------------------------------
Message: 2
Date: Fri, 18 Sep 2009 11:29:22 -0600
From: "Metzger, Kenneth" <kenneth.metzger <@t> aruplab.com>
Subject: [Histonet] Whole Mount Coverslips
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<FEE2FE3BA674F94A863BFC8F00D38ACB0C3089 <@t> postoffice01.aruplab.net>
Content-Type: text/plain; charset="us-ascii"
Can anyone tell me who sells whole mount coverslips besides Brain
Research Labs? They have mine back-ordered until 10/8/09 and I need some
before then. Thanks,
Ken
Kenneth G Metzger HTL(ASCP)
ARUP Labs
Histology Supervisor
500 Chipeta Way
Salt Lake City, Utah 84108-1221
kenneth.metzger <@t> aruplab.com
(801) 583-2787 x 3101
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------------------------------
Message: 3
Date: Fri, 18 Sep 2009 13:44:56 -0400
From: "Shirley A. Powell" <POWELL_SA <@t> mercer.edu>
Subject: [Histonet] RE: Whole Mount Coverslips
To: "Metzger, Kenneth" <kenneth.metzger <@t> aruplab.com>, "Histonet
(histonet <@t> lists.utsouthwestern.edu)"
<histonet <@t> lists.utsouthwestern.edu>
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<9BF995BC0E47744E9673A41486E24EE21B58759F3A <@t> MERCERMAIL.MercerU.local>
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Hi Kenneth,
Most any company that makes slides or coverslips can make you the size you need. Not sure how long it would take but there are more than one source. Try Thermo FIsher reps, they bought out Richard Alan who used to make any size needed. Erie who makes glass slides and coverslips I think was bought out by ehm too.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth
Sent: Friday, September 18, 2009 1:29 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Whole Mount Coverslips
Can anyone tell me who sells whole mount coverslips besides Brain
Research Labs? They have mine back-ordered until 10/8/09 and I need some
before then. Thanks,
Ken
Kenneth G Metzger HTL(ASCP)
ARUP Labs
Histology Supervisor
500 Chipeta Way
Salt Lake City, Utah 84108-1221
kenneth.metzger <@t> aruplab.com
(801) 583-2787 x 3101
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The information transmitted by this e-mail and any included
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and may constitute inside or non-public information under
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information and is intended only for the use of the recipient.
Unauthorized forwarding, printing, copying, distributing, or use of
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are not the intended recipient, please promptly delete this e-mail
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------------------------------
Message: 4
Date: Fri, 18 Sep 2009 10:49:54 -0700
From: Jennifer Anderson <janderson <@t> halozyme.com>
Subject: [Histonet] black plastic incubation boxes
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<5F7CC9B788911848A79BC83453A3D653028842D70D <@t> Tomlinson.hti.com>
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Hello Histonet!
I have a large black square molded plastic box ( about 15"x15"x2") that I use for IHC. It holds 30 slides (10 slides on 3 sticky rails). We have smaller boxes (15"x7"x2") of the same molded plastic that I can order from American Master Tech (holds 20 slides) but I want another large square box. Does anyone have an idea where I can find one?
Thanks a lot!
Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333
janderson <@t> halozyme.com<mailto:janderson <@t> halozyme.com>
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------------------------------
Message: 5
Date: Fri, 18 Sep 2009 14:08:22 -0400
From: Jack Ratliff <ratliffjack <@t> hotmail.com>
Subject: RE: [Histonet] Whole Mount Coverslips
To: <kenneth.metzger <@t> aruplab.com>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU108-W2642BAEE2268A80FE672D5AEE00 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
What size do you need?
Jack Ratliff
> Date: Fri, 18 Sep 2009 11:29:22 -0600
> From: kenneth.metzger <@t> aruplab.com
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Whole Mount Coverslips
>
> Can anyone tell me who sells whole mount coverslips besides Brain
> Research Labs? They have mine back-ordered until 10/8/09 and I need some
> before then. Thanks,
>
>
>
> Ken
>
>
>
> Kenneth G Metzger HTL(ASCP)
>
> ARUP Labs
>
> Histology Supervisor
>
> 500 Chipeta Way
>
> Salt Lake City, Utah 84108-1221
>
> kenneth.metzger <@t> aruplab.com
>
> (801) 583-2787 x 3101
>
>
>
>
> - ------------------------------------------------------------------
> The information transmitted by this e-mail and any included
> attachments are from ARUP Laboratories and are intended only for the
> recipient. The information contained in this message is confidential
> and may constitute inside or non-public information under
> international, federal, or state securities laws, or protected health
> information and is intended only for the use of the recipient.
> Unauthorized forwarding, printing, copying, distributing, or use of
> such information is strictly prohibited and may be unlawful. If you
> are not the intended recipient, please promptly delete this e-mail
> and notify the sender of the delivery error or you may call ARUP
> Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1
> (800) 522-2787 ext. 2100
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Fri, 18 Sep 2009 11:00:04 -1000
From: Hugh Luk <hlukey <@t> msn.com>
Subject: RE: [Histonet] Ultra-Low Temp. Upright Freezer
To: <lucie.s.guernsey <@t> gmail.com>, histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT106-W2A98EDAD7C4FE14AE57ABC3E00 <@t> phx.gbl>
Content-Type: text/plain; charset="Windows-1252"
Hi Lucie,
My research group has 63 Revco (or Harris-same brand) upright freezers of various ages at my location. All are 21 to 25 cu ft. Within my organization, there are about 200 on three campuses. The older ones break down with some regularity, (maybe 2/63 per year), so my biomed tech insists on Revco. We have not lost an upright freezer, and every unit that goes down has been repaired. We do not have any affiliation with Revco.
We have purchased 7 in the last 4 years, and I have to admit, 2 years ago, several new units were flawed (motors not in synch[?] and never attained -80C). However, the units were repaired and the newer units appear flawless.
Their alarms are adjustable (ie. "Warm point alarm") so we do not have problems with over or under sensitivity.
How loud? They are fairly loud, but tolerable as a moderate hum. We have several units interspersed throughout the labs, and these single units are fine. However, the thermal release from the motors in smaller room with multiple freezers (side-by-side) is significant and given these two factors in this scinerio, we keep the area around them clear.
Most of the hospitals I work with have Revco's too. Several are lay-down, but most are upright. The lay-down units seem to have fewer problems with frost, but take up a larger footprint. They have them in the work areas with seemingly no problems.
The Sanyo unit looks good and if my biomed tech would not have killed me (did not mean literally), I would have purchased them. I think they are less expensive and come from a dependable source (in my opinion). We just didn't know about replacement parts to my area.
Hope this helps and good luck with your search,
Hugh Luk, HTL (ASCP)
Pathology shared resource lab manager
Cancer research center of Hawaii
> From: lucie.s.guernsey <@t> gmail.com
> Date: Thu, 17 Sep 2009 18:15:15 -0700
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Ultra-Low Temp. Upright Freezer
>
> Hi all!
>
> I'm hoping to get some help RE: purchasing a new ultra-low temp. upright
> freezer. I've tried searching Histonet, but many of the posts are a few
> years old, and my guess is that the products have changed a bit over the
> years.
>
> My lab is looking to purchase a large (absolute minimum size of 17 cu.ft.,
> but preferably much larger), and definitely upright (not chest) -80.
> Unfortunately, since this is my first foray into the world of lab freezers,
> I know very little about them. I have discovered that Thermo's Revco and
> Sanyo freezers seem to be popular. Obviously we're looking for a freezer
> that is as large and efficient as possible. Insulation and the freezer's
> compressor(s) are most important.
>
> I've spoken with my Sanyo rep. and the VIP Series MDF-U73VC (26 cu.ft.)
> freezer seems quite nice - small exterior footprint and his sales pitch
> regarding the vacuum insulation (therefore denser insulation), insulated
> inner doors, and supposed minimized carbon footprint was quite convincing. I
> still plan on talking to Revco's rep. RE: their Elite and Ultima freezers,
> though my guess is that their sales pitch will be just as convincing.
>
> Since you can't depend only on sales pitches, I'm depending on colleagues as
> well.... Does anyone have any experience with either the Sanyo or Revco
> freezers (or any others that they'd like to recommend)? Any and all positive
> and negative feedback would be greatly appreciated! I'm hoping to get
> comments regarding:
> - efficiency
> - how often service is needed due to failures
> - are the alarms sensitive enough or maybe too sensitive
> - how loud the freezer is when the compressor is on
> - etc......
>
> As always, please remember to Reply All.
>
> Thank you in advance for your help! I look forward to hearing everyone's
> suggestions.
>
>
> Lucie
>
> UCSD
> lguernsey <@t> ucsd.edu
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> Histonet <@t> lists.utsouthwestern.edu
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