[Histonet] zinc fixation

[julia hough]

Hello. Histonetters. 

I have recently used zinc fixed tibia and calvaria for the
staining of CD31. My CD31 does seem to have worked, however there seems to be
marrow loss and digestion of the marrow also. 

 

I zinc fixed the bones for 48 hours and then used EDTA pH 7
for decalcification for 2 weeks. I am unsure whether I have fixed the bones for
too long, whether the decal has been affected by the zinc fix (as the bones
were “jelly-like” before processing) or whether the decalcification needs
shortening when using zinc fixation.

 

If anyone has any experience in using zinc fixation on tibia
for Immuno staining and can offer some advice that would be much appreciated.

 

Thanks

 

Julia


_________________________________________________________________
Learn how to add other email accounts to Hotmail in 3 easy steps.
http://clk.atdmt.com/UKM/go/167688463/direct/01/