[Histonet] Polarizing filters

Anthony Reilly Tony_Reilly <@t> health.qld.gov.au
Thu Sep 17 18:49:15 CDT 2009

I worked with a pathologist many years ago who would use one piece of polarising glass on the light source and wear his sunglasses.  It worked fine.
Tony Reilly

Chief Scientist
Anatomical Pathology
Pathology Queensland
Level 1, Building 15
Princess Alexandra Hospital
Ipswich Rd, 
Woolloongabba Q 4102
Ph: 07 32402412
Fax:07 32402930
tony_reilly <@t> health.qld.gov.au

>>> Eridana <eridana <@t> cox.net> 18/09/2009 3:49 am >>>
You can use glass camera filters.  At the store they had a huge supply of polarizing filters.  I bought 2 for about $20 each that were the same brand, but not even the same diameter.  I put one on top of the slide and one on the light source and it worked great.  I also rotated the light source filter since it was too easy to bump the slide when trying to rotate the upper one.

It was really interesting to see all the non collagen that was positive in the staining but not when polarized.

Donna Harclerode HT,HTL,QIHC (ASCP),SLS 
Histology Core Manager 
UCSD, Dept of Pathology 
9500 Gillman Drive 
BSB 2010 
San Diego, CA 92071 
858 534 7438

Date: Wed, 16 Sep 2009 14:12:38 -0400 
From: "Monfils, Paul" <PMonfils <@t> Lifespan.org> 
Subject: RE: [Histonet] Polarizing filters 
To: <histonet <@t> lists.utsouthwestern.edu> 
<4EBFF65383B74D49995298C4976D1D5E03835CE6 <@t> LSRIEXCH1.lsmaster.lifespan.org> 

Content-Type: text/plain;charset="iso-8859-1" 

The polarizer and analyzer are identical filters, and either of them can be used 
in either location. One must be between the light source and the slide being 
viewed. The other must be between the slide being viewed and your eye or camera.  
I place one filter directly on top of my illuminator.  The other is in a filter 
slide in the microscope column, which can be pushed into the light beam or 
pulled out of it, but you can also place it directly on top of the slide. You 
rotate either filter to achieve the polarization effect.  I rotate the lower one 
since the other one is not accessible.  These filters cut down the light 
intensity substantially, so you should use them with maximum brightness of the 
illuminator, iris diaphram wide open, and with neutral density or any other 
kinds of filters removed from the light beam, including the blue filter if you 
normally use one.  Polarizing filters can be purchased at any camera store, and 
some science supply companies sell them.  Get good quality glass filters though, 
not cheap plastic ones. 

> ---------- 
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of jstaruk 
> Sent: Thursday, September 10, 2009 4:46 PM 
> To: histonet <@t> lists.utsouthwestern.edu 
> Subject: [Histonet] Polarizing filters 
> Does anyone know where I can find the two appropriate filters (lenses) 
> needed to polarize the congo red and Sirius red stains?  I have an Olympus 
> CH-2 that needs to be fitted.  I understand I need a "polarizer" lens and an 
> "analyzer" lens.  Are these two different lenses or the same lens, just in 
> different locations on the microscope? 
> Thank you 
> Jim 
> _______________________ 
> James E. Staruk HT(ASCP) 
>  www.masshistology.com 
>   www.nehorselabs.com 

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