[Histonet] Re: Polarizing filters (jstaruk)

[Sarah Tarran]

Hi James,

We have a Leica inverted microscope and just use polarising filters that
are meant for cameras to look at our Sirius red stains (one is a cannon
58mm screw-in filter UV x1, the other we borrow from our audio-vision
department so I am not sure what it is). We tape one directly below the
stage and put one immediately above the slide. We then rotate the one
above the slide until we have a black background and the collagen is
shining yellow. If you get desperate, you can always use polarised
sunglasses to have a look at your slides - not ideal but good for a quick
look.

Hope this helps!

Sarah

>
>
> Message: 13
> Date: Thu, 10 Sep 2009 16:46:38 -0400
> From: "jstaruk" <jstaruk <@t> masshistology.com>
> Subject: [Histonet] Polarizing filters
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <ACC824B131034482B4E081D9F1AEBE9A <@t> JimPC>
> Content-Type: text/plain;	charset="us-ascii"
>
> Does anyone know where I can find the two appropriate filters (lenses)
> needed to polarize the congo red and Sirius red stains?  I have an Olympus
> CH-2 that needs to be fitted.  I understand I need a "polarizer" lens and
> an
> "analyzer" lens.  Are these two different lenses or the same lens, just in
> different locations on the microscope?
>
> Thank you
>
> Jim
>
> _______________________
> James E. Staruk HT(ASCP)
>  www.masshistology.com
>   www.nehorselabs.com