[Histonet] formalin storage

[Galbraith, Joe]

Additionally, we have been told that the reaction product is also a very
potent carcinogen and that is the reason why you are supposed to
thoroughly rinse tissue free of unbound formalin prior to immersion in
HCl based decalcification agents and once again when removing from decal
and placing the tissue back into formalin.  

Joe
joseph-galbraith <@t> uiowa.edu


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Smith,
Allen
Sent: Friday, September 11, 2009 3:29 PM
To: 'ti fei'
Cc: 'Histonet <@t> lists.utsouthwestern.edu'
Subject: RE: Re:[Histonet] formalin storage

Theoretically, the fumes of HCl can react with the formaldehyde fumes to
produce bis-chloromethyl ether, which is twice as toxic as osmium
tetroxide.  The yield from this reaction at 1 Atm is so small that I
don't think I would worry about it unless I had open containers in a
closed space.
Allen A. Smith
Professor of Anatomy 
Barry University School of Podiatric Medicine
Miami Shores, FL

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of ti fei
Sent: Friday, September 11, 2009 10:23 AM
To: Riesen, Rebecca; histonet
Subject: Re:[Histonet] formalin storage

never store everything in one cabinet.
 As far as i know, HCl and formalin should not be stored together. If
any of you know why...
   
 ------------------ Original ------------------
  From:  "Riesen, Rebecca"<Rebecca.Riesen <@t> nchmd.org>;
 Date:  Fri, Sep 11, 2009 10:09 PM
 To:  "histonet"<histonet <@t> lists.utsouthwestern.edu>; 
 
 Subject:  [Histonet] formalin storage

  
 
Thank you all! I have received many responses already concerning 10%
Neutral Buffered Formalin (NBF) storage.  Only one person has stated
that they store all chemicals, including formalin, in safety cabinets.
I just want to clarify something.  All MSDS's I studied stated that 10%
NBF indeed is NOT flammable, but the Flash Point is under 200 F on all
but one manufacturer's product.  The NFPA99 11.7.2.3.1 and 11.7.2.3.2
rules on flammables storage include all Class I, II and IIIA liquids.
Class IIIA liquids include those with a Flashpoint of less than 200 F.
That would include 10% NBF.  It appears to be the formaldehyde fume
(which we all know very well) is the combustible portion no matter if it
is 96.7% water. Most manufacturer's recommendations are for storage in a
tightly sealed container, probably to keep those nasty fumes inside.
One would think that would be sufficient. 
Riesen, Rebecca
Rebecca.Riesen <@t> nchmd.org
NCH Healthcare Systems
Direct 239-436-5000 x2188
Fax 239-436-6767


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