[Histonet] DAB and nuclear staining
Melissa Mazan
melissa.mazan <@t> tufts.edu
Thu Sep 3 08:18:38 CDT 2009
Hi all,
I have been having a problem with counterstaining for nuclei - I'm
looking at mouse lung tissues and staining macrophages with F4/80 (rat
anti-mouse). I use a negative serum control and a rat IgG isotype
control. I see very clear staining of macrophages - but when I
counterstain with hematoxylin or nuclear red, everything becomes muddy
and I can no longer discern the DAB staining. Can anyone offer advice? I
am using the Vector hematoxylin, and have tried as little as 5 seconds.
Melissa
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> Today's Topics:
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> 1. Re:Endogenous biotin blocking (Hobbs, Carl)
> 2. Marginated Staining on IHC (Katelin Lester)
> 3. Re: H&E preparation (Rene J Buesa)
> 4. Book specific for Neuropathology techniques/methods (Sharon Allen)
> 5. RE: H&E preparation (Tony Henwood)
> 6. Mast cell staining HELP! (Kim O'Sullivan)
> 7. RE: H&E preparation (Kemlo Rogerson)
> 8. RE: H&E preparation (Lynette Pavelich)
> 9. Ink issues (Amspacher, September)
> 10. RE: Ink issues (Walzer Susan)
> 11. RE: Gloves (Bonner, Janet)
> 12. RE: Ink issues (Smith Wanda)
> 13. RE: Ink issues (Weems, Joyce)
> 14. RE: H&E preparation (Weems, Joyce)
> 15. Robotic Prostatectomy (Phyllis Thaxton)
> 16. (no subject) (James, Jennifer)
> 17. Cost to produce an H&E slide (Amy Johnson)
> 18. Re: Robotic Prostatectomy (Rene J Buesa)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 1 Sep 2009 19:23:46 +0100
> From: "Hobbs, Carl" <carl.hobbs <@t> kcl.ac.uk>
> Subject: [Histonet] Re:Endogenous biotin blocking
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <11D9615B89C10747B1C985966A63D7CA2991742A1D <@t> KCL-MAIL04.kclad.ds.kcl.ac.uk>
>
> Content-Type: text/plain; charset="us-ascii"
>
>
> Your understanding is correct, imho.
> I add a "no primary" control on my stABCpx-DAB run whenever I am testing new tissues and assess any positivity that could be due to endogenous biotin.
> You will quickly build up a knowledge of those tissues that automatically require biotin - blocking. ( eg liver, kidney)
> If you are a clinical/registered Lab, you wil of course be required to buy a commercial kit.
> If you are in a research lab and find that you will need to apply biotin blocking regularly, you can make up your own avidin/biotin solutions if costs are a major factor.
> I will be interested in other suggestions/approaches.
> carl
>
>
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Tue, 1 Sep 2009 11:47:22 -0700
> From: "Katelin Lester" <katelin <@t> cuttingedgehistology.com>
> Subject: [Histonet] Marginated Staining on IHC
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <F4175DFDCB414F1BA1F2AD3E3FFEFBF3 <@t> Front>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi histonet,
>
> I am back with a problem I've been having with my IHC slides. We had a PM
> recently and had thought that would solve this problem, but as I do more and
> more slides, the problem remains. I am seeing marginated staining at the
> edge of the tissue on the controls as well as the patient tissue. This
> occurs at the top, middle, and bottom of the slides. This occurs with any
> antibody. This does not occur with every run, but the more slides I have on
> the stainer, the more frequently it occurs. I have contacted Thermo, who
> now takes care of our old R.A.S. Microm HMS 710i, but I have further faith
> that the histonet can help me. I've seen similar posts in the archives, but
> did not see any responses.
>
> Any suggestions are appreciated,
>
> Katelin
>
>
>
> Katelin Lester
>
> Cutting Edge Histology Services, LLC
>
> (503) 443-2157
>
>
>
>
>
> ------------------------------
>
> Message: 3
> Date: Tue, 1 Sep 2009 12:22:06 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] H&E preparation
> To: histonet <@t> lists.utsouthwestern.edu, Aazath Raj <aazath <@t> hotmail.com>
> Message-ID: <960806.99145.qm <@t> web65709.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=utf-8
>
> The hematoxylin has to be ripen, either naturally (it takes long time) or with an oxidizer.
> René J.
>
> --- On Tue, 9/1/09, Aazath Raj <aazath <@t> hotmail.com> wrote:
>
>
> From: Aazath Raj <aazath <@t> hotmail.com>
> Subject: [Histonet] H&E preparation
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Tuesday, September 1, 2009, 11:48 AM
>
>
>
> Dear All,
> Â Â Â Â Â We are preparing harris heamtoxylin for H&E in our laboratory. some time we get intense nuclear staining some time light weak.Does anybody know how to control a consistency in the strength .
>
>
> Aazath
> Technical Officer
> Apollo Hospitals
> India
>
> _________________________________________________________________
> News, sports, entertainment and fine living…learn the ropes on MSN India
> http://in.msn.com_______________________________________________
> Histonet mailing list
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Tue, 1 Sep 2009 16:14:18 -0500
> From: "Sharon Allen" <SAllen <@t> exchange.hsc.mb.ca>
> Subject: [Histonet] Book specific for Neuropathology
> techniques/methods
> To: <histonet <@t> pathology.swmed.edu>
> Message-ID:
> <BB6ADCD4B7ABB045A09A7634AC15CC610BEC8092 <@t> hscxmsmx0010.ad.wrha.mb.ca>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi,
> Can anyone on the Histonet recommend a good book of Neuropathology
> techniques/methods for use in a Neuropathology Lab. Need more up to
> date reference material.
> Thanks
> Sharon Allen
> Neuropathology Lab
> HSC - Wpg
> sallen <@t> hsc.mb.ca
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>
> ------------------------------
>
> Message: 5
> Date: Wed, 2 Sep 2009 10:21:20 +1000
> From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
> Subject: RE: [Histonet] H&E preparation
> To: "Aazath Raj" <aazath <@t> hotmail.com>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <B9EAF61856077F47BF9BE2F89AFC55520685376B <@t> hedwig.nch.kids>
> Content-Type: text/plain; charset="us-ascii"
>
> Aazath,
>
> Several possibles:
> It can come down to the ripening time of the haematoxylin.
> Hx with rapid ripening (more oxidiser, sodium iodite or (heaven forbid!)
> mercury oxide, added) will tend to stain strongly initially but tend to
> over-oxidise giving weak or brown nuclear staining.
>
> Heating the solution and adding the oxidiser will cause the same thing.
>
> Less oxidiser will give you a solution with a longer self life but
> weaker staining.
>
> If the pH is too low, staining will be weaker, but more specific.
>
>
> Regards
>
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
> Laboratory Manager & Senior Scientist
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Aazath
> Raj
> Sent: Wednesday, 2 September 2009 1:49 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] H&E preparation
>
>
>
> Dear All,
> We are preparing harris heamtoxylin for H&E in our laboratory.
> some time we get intense nuclear staining some time light weak.Does
> anybody know how to control a consistency in the strength .
>
>
> Aazath
> Technical Officer
> Apollo Hospitals
> India
>
> _________________________________________________________________
> News, sports, entertainment and fine living...learn the ropes on MSN
> India http://in.msn.com_______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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>
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>
>
>
> ------------------------------
>
> Message: 6
> Date: Wed, 02 Sep 2009 12:33:52 +1000
> From: Kim O'Sullivan <Kim.Osullivan <@t> med.monash.edu.au>
> Subject: [Histonet] Mast cell staining HELP!
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <130.194.114.97.1251858518 <@t> my.monash.edu.au>
> Content-Type: text/plain; charset=UTF-8
>
> Hi,
>
> Can anyone out there recommend the best way to stain mouse mast cells in the kidney(toluidine blue/or berberine sulfate) with a protocol that produces cconsistent results?
>
> Secondly, I have mouse kidney sections fixed in FFPE, PLP and methyl Carnoys. Does anyone know of a company that supplies antibodies which will work on any of these fixatives,for the staining of the IgE receptor, mast cell trytptase and chymase.
>
> Any advice would be appreciated!
>
>
> Kim O'Sullivan
> Monash University
> Melbourne
> Australia
>
>
>
> ------------------------------
>
> Message: 7
> Date: Wed, 2 Sep 2009 08:53:46 +0100
> From: "Kemlo Rogerson" <Kemlo.Rogerson <@t> waht.swest.nhs.uk>
> Subject: RE: [Histonet] H&E preparation
> To: "Aazath Raj" <aazath <@t> hotmail.com>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <86ADE4EB583CE64799A9924684A0FBBF077273FD <@t> wahtntex2.waht.swest.nhs.uk>
> Content-Type: text/plain; charset="us-ascii"
>
> Ripening in the sun (we do get some in the UK) used to give Haematoxylin
> that was stable; if you forgot to make any up then you hastely added
> oxidising agent to a new batch or 'jumped started' a batch that was as
> yet still immature. That batch then never seemed to last as long as you
> got muddy staining. They looked quite pretty, lines of 5 litres flasks
> with their contents ripening in the sun with cotton wool balls sticking
> out of the top, next to the cigarette ashtrays. How things have
> changed..............
>
>
>
>
>
>
>
>
> Kemlo Rogerson
> e-mail kemlorogerson <@t> nhs.net if not at work.
> DD 01934 647057 or extension 3311 Mob 07749 754194;
> Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah
> Lehrer
> This e-mail is confidential and privileged. If you are not the intended
> recipient please accept my apologies; please do not disclose, copy or
> distribute information in this e-mail or take any action in reliance on
> its contents: to do so is strictly prohibited and may be unlawful.
> Please inform me that this message has gone astray before deleting it.
> Thank you for your co-operation
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Aazath
> Raj
> Sent: 01 September 2009 16:49
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] H&E preparation
>
>
> Dear All,
> We are preparing harris heamtoxylin for H&E in our laboratory.
> some time we get intense nuclear staining some time light weak.Does
> anybody know how to control a consistency in the strength .
>
>
> Aazath
> Technical Officer
> Apollo Hospitals
> India
>
> _________________________________________________________________
> News, sports, entertainment and fine living...learn the ropes on MSN
> India http://in.msn.com_______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 8
> Date: Wed, 02 Sep 2009 07:01:39 -0400
> From: "Lynette Pavelich" <lpaveli1 <@t> hurleymc.com>
> Subject: RE: [Histonet] H&E preparation
> To: <aazath <@t> hotmail.com>,<histonet <@t> lists.utsouthwestern.edu>,
> <Kemlo.Rogerson <@t> waht.swest.nhs.uk>
> Message-ID: <4A9E1853020000EE0002C151 <@t> smtp-gw.hurleymc.com>
> Content-Type: text/plain; charset=US-ASCII
>
> Ah, the good 'ol days! Remember adding the oxidizinng agent (mecuric
> oxide) too soon when it was still bubbling and having a volcano of
> hematoxylin?!!! LOLj
> Was remembering tho', that every day, before using, we would "top off"
> (add...oh, about 30ml) of fresh to the filtered used batch. This really
> kept the quality good. Worked for 30 yrs until we started purchasing
> it. Gone are the morning coffee and donuts sitting next to our
> microtomes too!! Sigh................ Ya, ya....I know!!!
>
>
>>>> "Kemlo Rogerson" <Kemlo.Rogerson <@t> waht.swest.nhs.uk> 09/02/09 3:53 AM
>>>>
>>>>
> Ripening in the sun (we do get some in the UK) used to give Haematoxylin
> that was stable; if you forgot to make any up then you hastely added
> oxidising agent to a new batch or 'jumped started' a batch that was as
> yet still immature. That batch then never seemed to last as long as you
> got muddy staining. They looked quite pretty, lines of 5 litres flasks
> with their contents ripening in the sun with cotton wool balls sticking
> out of the top, next to the cigarette ashtrays. How things have
> changed..............
>
>
>
>
>
>
>
>
> Kemlo Rogerson
> e-mail kemlorogerson <@t> nhs.net if not at work.
> DD 01934 647057 or extension 3311 Mob 07749 754194;
> Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah
> Lehrer
> This e-mail is confidential and privileged. If you are not the intended
> recipient please accept my apologies; please do not disclose, copy or
> distribute information in this e-mail or take any action in reliance on
> its contents: to do so is strictly prohibited and may be unlawful.
> Please inform me that this message has gone astray before deleting it.
> Thank you for your co-operation
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Aazath
> Raj
> Sent: 01 September 2009 16:49
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] H&E preparation
>
>
> Dear All,
> We are preparing harris heamtoxylin for H&E in our laboratory.
> some time we get intense nuclear staining some time light weak.Does
> anybody know how to control a consistency in the strength .
>
>
> Aazath
> Technical Officer
> Apollo Hospitals
> India
>
> _________________________________________________________________
> News, sports, entertainment and fine living...learn the ropes on MSN
> India http://in.msn.com_______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 9
> Date: Wed, 2 Sep 2009 07:02:30 -0400
> From: "Amspacher, September" <september.amspacher <@t> bassett.org>
> Subject: [Histonet] Ink issues
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <71A87F8FEFFB024DB88B064543C7F24B13283139 <@t> ex2.bassett.org>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Good Morning all- I am having issues getting ink to "stick" to the tissue surface, the black and blue that we are using are fine we have tried several different companies and even use a "ink stay" acetic acid spray on the tissue. My new pathologist has talked about dipping the tissue after it is inked into Bouins solution, and that they didn't have any issues with inks where he came from. I have heard of this technique before, But I was just able to get rid of all of the bounins solutions in the lab and I am hesitant to bring it back, looking for Ideas and thought from all, also if you use the Bouins solution to "fix" your inks- I would love to learn your thoughts about the process. Thanks-a-million
>
> September Amspacher HT(ASCP)
> Technical Specialist- Histology Department
> Laboratory Chemical Hygiene Officer
> Bassett Healthcare
> Cooperstown, New York
>
>
>
>
>
> ------------------------------
>
> Message: 10
> Date: Wed, 2 Sep 2009 07:18:22 -0500
> From: Walzer Susan <Susan.Walzer <@t> HCAHealthcare.com>
> Subject: [Histonet] RE: Ink issues
> To: "Amspacher, September" <september.amspacher <@t> bassett.org>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <4BF03F5404EBDE409AF9232DA74B9DED2AC41FD3CD <@t> FWDCWPMSGCMS09.hca.corpad.net>
>
> Content-Type: text/plain; charset="us-ascii"
>
> My Docs use acetone.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amspacher, September
> Sent: Wednesday, September 02, 2009 7:03 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Ink issues
>
> Good Morning all- I am having issues getting ink to "stick" to the tissue surface, the black and blue that we are using are fine we have tried several different companies and even use a "ink stay" acetic acid spray on the tissue. My new pathologist has talked about dipping the tissue after it is inked into Bouins solution, and that they didn't have any issues with inks where he came from. I have heard of this technique before, But I was just able to get rid of all of the bounins solutions in the lab and I am hesitant to bring it back, looking for Ideas and thought from all, also if you use the Bouins solution to "fix" your inks- I would love to learn your thoughts about the process. Thanks-a-million
>
> September Amspacher HT(ASCP)
> Technical Specialist- Histology Department
> Laboratory Chemical Hygiene Officer
> Bassett Healthcare
> Cooperstown, New York
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 11
> Date: Wed, 2 Sep 2009 09:10:42 -0400
> From: "Bonner, Janet" <Janet.Bonner <@t> FLHOSP.ORG>
> Subject: RE: [Histonet] Gloves
> To: "Merced M Leiker" <leiker <@t> buffalo.edu>, "Mahoney,Janice A"
> <Janice.Mahoney <@t> alegent.org>, "Histonet"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <5F31F38C96781A4FBE3196EBC22D47807F2B1E <@t> fhosxchmb006.ADVENTISTCORP.NET>
>
> Content-Type: text/plain; charset=iso-8859-1
>
> We use Aloe Touch Nitrile gloves, powder-free from Medline (MDS195084 for small size) www.medline.com. (1-800 - medline) These gloves are not stiff, they fit the hand 'like a glove' . They say on the box "not intended to be used as a chemical barrier", but they do a great job when exposed to Histology chemicals.
>
> Janet
>
> ________________________________
>
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Merced M Leiker
> Sent: Mon 8/31/2009 12:43 PM
> To: Mahoney,Janice A; 'Histonet'
> Subject: Re: [Histonet] Gloves
>
>
>
> I would like to know the answer to this one too. I've been using nitrile
> gloves, which are rated as having moderate protection against xylene (they
> slow down how quickly xylene goes through them?) The brand I use is what's
> in our stockroom: Kimberly Clark and they come in a pretty purple. :-)
>
>
> --On Monday, August 31, 2009 11:15 AM -0500 "Mahoney,Janice A"
> <Janice.Mahoney <@t> alegent.org> wrote:
>
>
>> I'd like some advice about gloves. What brand/vendor are people using
>> that are approved for use with xylene? Thanks,
>> Jan Mahoney
>> Omaha
>>
>> ________________________________
>> Sponsored by Catholic Health Initiatives and Immanuel Health Systems,
>> Alegent Health is faithful to the healing ministry of Jesus Christ,
>> providing high quality care for the body, mind and spirit of every person.
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>
>
>
> Merced M Leiker
> Research Technician II
> Cardiovascular Medicine
> 348 Biomedical Research Building
> State University of New York at Buffalo
> 3435 Main St, Buffalo, NY 14214 USA
> leiker <@t> buffalo.edu
> 716-829-6118 (Ph)
> 716-829-2665 (Fx)
>
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>
>
> =======================================================
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> ------------------------------
>
> Message: 12
> Date: Wed, 2 Sep 2009 09:07:02 -0500
> From: Smith Wanda <Wanda.Smith <@t> HCAhealthcare.com>
> Subject: [Histonet] RE: Ink issues
> To: "Amspacher, September" <september.amspacher <@t> bassett.org>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <9E2D36CE2D7CBA4A94D9B22E8328A3BAFC79639B <@t> NADCWPMSGCMS03.hca.corpad.net>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Good Morning,
> We dip the inked tissue in a container of Acetone. We use the 7-dye kit from Bradley Products, Inc.
> Wanda
>
>
> WANDA G. SMITH, HTL(ASCP)HT
> Pathology Supervisor
> TRIDENT MEDICAL CENTER
> 9330 Medical Plaza Drive
> Charleston, SC 29406
> 843-847-4586
> 843-847-4296 fax
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amspacher, September
> Sent: Wednesday, September 02, 2009 7:03 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Ink issues
>
> Good Morning all- I am having issues getting ink to "stick" to the tissue surface, the black and blue that we are using are fine we have tried several different companies and even use a "ink stay" acetic acid spray on the tissue. My new pathologist has talked about dipping the tissue after it is inked into Bouins solution, and that they didn't have any issues with inks where he came from. I have heard of this technique before, But I was just able to get rid of all of the bounins solutions in the lab and I am hesitant to bring it back, looking for Ideas and thought from all, also if you use the Bouins solution to "fix" your inks- I would love to learn your thoughts about the process. Thanks-a-million
>
> September Amspacher HT(ASCP)
> Technical Specialist- Histology Department Laboratory Chemical Hygiene Officer Bassett Healthcare Cooperstown, New York
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 13
> Date: Wed, 2 Sep 2009 10:09:55 -0400
> From: "Weems, Joyce" <JWeems <@t> sjha.org>
> Subject: RE: [Histonet] Ink issues
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <5D64396A0D4A5346BEBC759022AAEAA57C2B89 <@t> ITSSSXM01V6.one.ads.che.org>
> Content-Type: text/plain; charset="us-ascii"
>
> We use acetic acid - 3-5% solution (vinegar). j
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Amspacher, September
> Sent: Wednesday, September 02, 2009 07:03
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Ink issues
>
> Good Morning all- I am having issues getting ink to "stick" to the
> tissue surface, the black and blue that we are using are fine we have
> tried several different companies and even use a "ink stay" acetic acid
> spray on the tissue. My new pathologist has talked about dipping the
> tissue after it is inked into Bouins solution, and that they didn't have
> any issues with inks where he came from. I have heard of this technique
> before, But I was just able to get rid of all of the bounins solutions
> in the lab and I am hesitant to bring it back, looking for Ideas and
> thought from all, also if you use the Bouins solution to "fix" your
> inks- I would love to learn your thoughts about the process.
> Thanks-a-million
>
> September Amspacher HT(ASCP)
> Technical Specialist- Histology Department Laboratory Chemical Hygiene
> Officer Bassett Healthcare Cooperstown, New York
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> Confidentiality Notice:
> This email, including any attachments is the
> property of Catholic Health East and is intended
> for the sole use of the intended recipient(s).
> It may contain information that is privileged and
> confidential. Any unauthorized review, use,
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> not the intended recipient, please reply to the
> sender that you have received the message in
> error, then delete this message.
>
>
>
>
> ------------------------------
>
> Message: 14
> Date: Wed, 2 Sep 2009 10:14:10 -0400
> From: "Weems, Joyce" <JWeems <@t> sjha.org>
> Subject: RE: [Histonet] H&E preparation
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <5D64396A0D4A5346BEBC759022AAEAA57C2B8E <@t> ITSSSXM01V6.one.ads.che.org>
> Content-Type: text/plain; charset="us-ascii"
>
>
> the good ole days for sure - purple ceilings, purple walls, purple
> people.... And cigarette ashes in the trash and food on the counter...
>
> Is it Friday yet?!!!
> J:>)
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lynette
> Pavelich
> Sent: Wednesday, September 02, 2009 07:02
> To: aazath <@t> hotmail.com; histonet <@t> lists.utsouthwestern.edu;
> Kemlo.Rogerson <@t> waht.swest.nhs.uk
> Subject: RE: [Histonet] H&E preparation
>
> Ah, the good 'ol days! Remember adding the oxidizinng agent (mecuric
> oxide) too soon when it was still bubbling and having a volcano of
> hematoxylin?!!! LOLj Was remembering tho', that every day, before
> using, we would "top off"
> (add...oh, about 30ml) of fresh to the filtered used batch. This really
> kept the quality good. Worked for 30 yrs until we started purchasing
> it. Gone are the morning coffee and donuts sitting next to our
> microtomes too!! Sigh................ Ya, ya....I know!!!
> Confidentiality Notice:
> This email, including any attachments is the
> property of Catholic Health East and is intended
> for the sole use of the intended recipient(s).
> It may contain information that is privileged and
> confidential. Any unauthorized review, use,
> disclosure, or distribution is prohibited. If you are
> not the intended recipient, please reply to the
> sender that you have received the message in
> error, then delete this message.
>
>
>
>
> ------------------------------
>
> Message: 15
> Date: Wed, 2 Sep 2009 08:26:06 -0700 (PDT)
> From: Phyllis Thaxton <dchihc <@t> yahoo.com>
> Subject: [Histonet] Robotic Prostatectomy
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <21877.6306.qm <@t> web43503.mail.sp1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> For the first time here, we have Urologists using the robotic method for removing prostates. The PA always receives them fresh, inks them, then puts the whole prostate in alcoholic fixative (currently Zfix from Anatech). The next day they are grossed in and processed. The prostates we have received lately are raw in the middle and unable to be grossed in, whereas before they were fine.
>
> Has anyone had similar experiences?
> Phyllis Thaxton HT(ASCP)QIHC
> DCH Regional Medical Center
> Tuscaloosa, AL
>
>
>
>
> ------------------------------
>
> Message: 16
> Date: Wed, 2 Sep 2009 10:54:28 -0500
> From: "James, Jennifer" <JamesJenniferD <@t> uams.edu>
> Subject: [Histonet] (no subject)
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <22AD9C7B0B022246AEF93ECF29BDB0D40B317D617E <@t> MAIL2.ad.uams.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> Does anyone have experience with the Cell Technology Apo-single stranded DNA antibody? Please let me know if you do.
> Thanks!
>
> Jennifer James BS, HT(ASCP)HTL, QIHC, CRS
> Research Assistant
> Experimental Pathology Laboratory
> Winthrop P.Rockefeller Cancer Institute, Room 429
> Universtiy of Arkansas for Medical Sciences
> 4301 West Markham Street, #725
> Little Rock, AR 72205
> 501-686-8265
> jamesjenniferd <@t> uams.edu<mailto:jamesjenniferd <@t> uams.edu>
>
>
>
>
> ------------------------------
>
> Message: 17
> Date: Wed, 2 Sep 2009 11:11:50 -0500
> From: "Amy Johnson" <AJohnson <@t> aipathology.com>
> Subject: [Histonet] Cost to produce an H&E slide
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <704247D5A09D004C9E6B115138D1703A07329E <@t> hpserv001.aipathology.local>
> Content-Type: text/plain; charset="US-ASCII"
>
> Hello Histonet.....Has anyone ever figured out how much it costs to
> produce an H&E slide? Are there any articles out there that would help
> to figure this out?
>
> I realize it may be different for each institution but a ball park
> figure would be greatly appreciated.
>
> Thanks
>
> Amylin Johnson
>
>
>
> ------------------------------
>
> Message: 18
> Date: Wed, 2 Sep 2009 09:51:25 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Robotic Prostatectomy
> To: histonet <@t> lists.utsouthwestern.edu, Phyllis Thaxton
> <dchihc <@t> yahoo.com>
> Message-ID: <909608.59241.qm <@t> web65704.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> And your problem will not be solved unless the prostates, after being inked, are cut in half before placing them in the fixative. I do not see any problem in cutting in half a properly inked prostate to assure proper fixation and subsequent processing.
> René J.
>
> --- On Wed, 9/2/09, Phyllis Thaxton <dchihc <@t> yahoo.com> wrote:
>
>
> From: Phyllis Thaxton <dchihc <@t> yahoo.com>
> Subject: [Histonet] Robotic Prostatectomy
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Wednesday, September 2, 2009, 11:26 AM
>
>
> For the first time here, we have Urologists using the robotic method for removing prostates. The PA always receives them fresh, inks them, then puts the whole prostate in alcoholic fixative (currently Zfix from Anatech). The next day they are grossed in and processed. The prostates we have received lately are raw in the middle and unable to be grossed in, whereas before they were fine.
>
> Has anyone had similar experiences?
> Phyllis Thaxton HT(ASCP)QIHC
> DCH Regional Medical Center
> Tuscaloosa, AL
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 70, Issue 2
> ***************************************
>
--
Melissa R. Mazan, DVM, Diplomate ACVIM
Associate Professor and Director of Equine Sports Medicine
Department of Clinical Sciences
Tufts Cummings School of Veterinary Medicine
200 Westborough Road
North Grafton,MA 01536
tel: 508-839-5395
fax: 508-839-7903
email: melissa.mazan <@t> tufts.edu
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