[Histonet] Ink issues (UNCLASSIFIED)

Gladney, Diane C Ms CIV USA MEDCOM MACH diane.gladney <@t> us.army.mil
Wed Sep 2 13:41:32 CDT 2009

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We use "Distilled White Vinegar". I buy it at the grocery store by the
gallon. It is the acetic acid in bouin's solution that fixes the ink to
the tissue, not the picric acid. I have been using white vinegar for
more years than I care to remember. Hope this helps.

Diane Gladney
Supervisor, Histology Dept.
Moncrief Army Community Hospital
Ft. Jackson, SC

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Amspacher, September
Sent: Wednesday, September 02, 2009 7:03 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Ink issues

Good Morning all- I am having issues getting ink to "stick" to the
tissue surface, the black and blue that we are using are fine we have
tried several different companies and even use a "ink stay" acetic acid
spray on the tissue. My new pathologist has talked about dipping the
tissue after it is inked into Bouins solution, and that they didn't have
any issues with inks where he came from.  I have heard of this technique
before, But I was just able to get rid of all of the bounins solutions
in the lab and I am hesitant to bring it back, looking for Ideas and
thought from all, also if you use the Bouins solution to "fix" your
inks- I would love to learn your thoughts about the process.

September Amspacher HT(ASCP)
Technical Specialist- Histology Department
Laboratory Chemical Hygiene Officer
Bassett Healthcare
Cooperstown, New York

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