[Histonet] Re: Ink issues

Robert Richmond rsrichmond <@t> gmail.com
Wed Sep 2 12:24:06 CDT 2009

About what to put on tissue to make the marking ink stick to it:

I don't use anything. The trick is to blot the surfaces thoroughly dry
with a paper towel before you try to put the ink on. Of course,
there's nothing you can use that will make ink adhere well to
cauterized surfaces (some breast biopsy specimens, LEEP cones, and
such. The pathologist can however identify cauterized margins under
the microscope.)

Because of the picric acid (and because it stains your clothes)
Bouin's fixative is unacceptable. So is acetone, which is a serious
explosion hazard. If you must use something, use 3% acetic acid (white
vinegar diluted half-strength).

I've been grossing since 1965, worked on maybe sixty different
pathology services.

Bob Richmond
Samurai Pathologist
Knoxville TN

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