[Histonet] RE: Ink issues

[Smith Wanda]

Good Morning,
We dip the inked tissue in a container of Acetone.  We use the 7-dye kit from Bradley Products, Inc. 
Wanda 


WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amspacher, September
Sent: Wednesday, September 02, 2009 7:03 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Ink issues

Good Morning all- I am having issues getting ink to "stick" to the tissue surface, the black and blue that we are using are fine we have tried several different companies and even use a "ink stay" acetic acid spray on the tissue. My new pathologist has talked about dipping the tissue after it is inked into Bouins solution, and that they didn't have any issues with inks where he came from.  I have heard of this technique before, But I was just able to get rid of all of the bounins solutions in the lab and I am hesitant to bring it back, looking for Ideas and thought from all, also if you use the Bouins solution to "fix" your inks- I would love to learn your thoughts about the process. Thanks-a-million

September Amspacher HT(ASCP)
Technical Specialist- Histology Department Laboratory Chemical Hygiene Officer Bassett Healthcare Cooperstown, New York



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