[Histonet] RE: Ink issues

Walzer Susan Susan.Walzer <@t> HCAHealthcare.com
Wed Sep 2 07:18:22 CDT 2009

My Docs use acetone.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amspacher, September
Sent: Wednesday, September 02, 2009 7:03 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Ink issues

Good Morning all- I am having issues getting ink to "stick" to the tissue surface, the black and blue that we are using are fine we have tried several different companies and even use a "ink stay" acetic acid spray on the tissue. My new pathologist has talked about dipping the tissue after it is inked into Bouins solution, and that they didn't have any issues with inks where he came from.  I have heard of this technique before, But I was just able to get rid of all of the bounins solutions in the lab and I am hesitant to bring it back, looking for Ideas and thought from all, also if you use the Bouins solution to "fix" your inks- I would love to learn your thoughts about the process. Thanks-a-million

September Amspacher HT(ASCP)
Technical Specialist- Histology Department
Laboratory Chemical Hygiene Officer
Bassett Healthcare
Cooperstown, New York

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