[Histonet] H&E preparation

Tony Henwood AnthonyH <@t> chw.edu.au
Tue Sep 1 19:21:20 CDT 2009


Several possibles:
It can come down to the ripening time of the haematoxylin.
Hx with rapid ripening (more oxidiser, sodium iodite or (heaven forbid!)
mercury oxide, added) will tend to stain strongly initially but tend to
over-oxidise giving weak or brown nuclear staining.

Heating the solution and adding the oxidiser will cause the same thing.

Less oxidiser will give you a solution with a longer self life but
weaker staining.

If the pH is too low, staining will be weaker, but more specific.


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Aazath
Sent: Wednesday, 2 September 2009 1:49 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] H&E preparation

Dear All,
          We are preparing harris heamtoxylin for H&E in our laboratory.
some time we get intense nuclear staining some time light weak.Does
anybody know how to control a consistency in the strength .

Technical Officer
Apollo Hospitals

News, sports, entertainment and fine living...learn the ropes on MSN
India http://in.msn.com_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.

More information about the Histonet mailing list