[Histonet] H&E preparation

[Tony Henwood]

Aazath,

Several possibles:
It can come down to the ripening time of the haematoxylin.
Hx with rapid ripening (more oxidiser, sodium iodite or (heaven forbid!)
mercury oxide, added) will tend to stain strongly initially but tend to
over-oxidise giving weak or brown nuclear staining.

Heating the solution and adding the oxidiser will cause the same thing.

Less oxidiser will give you a solution with a longer self life but
weaker staining.

If the pH is too low, staining will be weaker, but more specific.


Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Aazath
Raj
Sent: Wednesday, 2 September 2009 1:49 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] H&E preparation



Dear All,
          We are preparing harris heamtoxylin for H&E in our laboratory.
some time we get intense nuclear staining some time light weak.Does
anybody know how to control a consistency in the strength .


Aazath
Technical Officer
Apollo Hospitals
India

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