[Histonet] Canine endothelial antibody

[Jan Shivers]

Jackie,

Dako's Factor VIII (von Willebrand Factor), cat. #A0082 works for me on 
canine tissue (and cat, pig, cow, horse, sheep, chicken, mouse, etc.).  I 
use Proteinase K enzyme digestion pretreatment for 5' at room temperature; 
primary rabbit Ab incubation 30', EnVision+/HRP anti-Rabbit 30' (with 4% 
normal dog serum added per volume for blocking), AEC 10'.

Hope this helps,

Jan Shivers
Senior Scientist
Pathology Teaching Program
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003 <@t> umn.edu

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----- Original Message ----- 
From: "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com>
To: <histonet <@t> lists.utsouthwestern.edu>; 
<histonet-bounces <@t> lists.utsouthwestern.edu>
Sent: Wednesday, October 28, 2009 3:35 PM
Subject: [Histonet] Canine endothelial antibody


>A week or so ago I thought I saw a post about an antibody that works for
> canine endothelial cells, but I can't find it in the archives - was I just
> dreaming, because I need this so bad?   I've tried Factor VIII and a CD34
> on FFPE, but with poor results.   Any help would be most appreciated.
> Thanks,
> Jackie O'
>
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