[Histonet] Canine endothelial antibody

Amy Porter portera <@t> msu.edu
Thu Oct 29 07:11:33 CDT 2009


We use Dako Von Willebrand Factor VIII in Rabbit on multiple species and it 
works fabulously.  Enzyme pretreatment with 0.03% pronase e for 10 mins at 
37C / Primary at 1:200 to 1:500 depending on the species being stained.
Amy S. Porter, HT (ASCP) QIHC
Investigative HistoPathology Laboratory - Supervisor
2201 Biomedical Physical Sciences Bldg.  Rm #2133
East Lansing, MI  48824-3320
Phone:  (517) 884-5026
Fax:  (517) 432-1368
Email:  portera <@t> msu.edu
Web:  www.humanpathology.msu.edu
----- Original Message ----- 
From: "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com>
To: <histonet <@t> lists.utsouthwestern.edu>; 
<histonet-bounces <@t> lists.utsouthwestern.edu>
Sent: Wednesday, October 28, 2009 4:35 PM
Subject: [Histonet] Canine endothelial antibody


>A week or so ago I thought I saw a post about an antibody that works for
> canine endothelial cells, but I can't find it in the archives - was I just
> dreaming, because I need this so bad?   I've tried Factor VIII and a CD34
> on FFPE, but with poor results.   Any help would be most appreciated.
> Thanks,
> Jackie O'
>
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