[Histonet] a basic question about immunohistochemistry

[Lesley Weston]

Just about all biology research institutions have an animal unit with  
qualified technicians to look after the animals, rather than someone  
qualified in other areas; however the technicians are subject to  
human failings such as going home at night. I've never heard of a one- 
person research team, and I don't think it would be all that effective.

	The tissue from the animal that died at the wrong time is not  
comparable to perfused tissue, but as Tom says, it might still be  
worth processing. If nothing else, it will show how much difference  
perfusion makes.

Lesley Weston.


On 28-Nov-09, at 10:17 AM, Thomas Jasper wrote:

> Dear Salim,
>
> As you have been informed, doing immunohistochemistry is possible on
> this tissue.  After all it's possible to do IHC on any tissue whether
> the conditions you want to test under are ideal or not.
>
> Being chastised on this list and calling your work "bad science" is
> totally out of line and certainly does not help you out.  I think some
> people would do well to reserve judgment, particularly when there's no
> way they can fully understand what's going on with your project.   
> Having
> worked in research myself, I completely understand that animals will
> die, at the most inconvenient times, during a study.
>
> First of all you should incorporate the data about the animal dying  
> into
> your study notes.  Secondly, there's no harm in running the IHC on  
> this
> animal's tissue.  You can use the results comparatively with results
> from some perfused tissue later on.
>
> I don't know Salim, some people might call it damage control, or  
> making
> the best of a less than ideal situation.  Again, I don't know exactly
> what you're working on but it seems there's information worth  
> gathering
> despite the circumstances.  I also understand that it's probably  
> next to
> impossible to carry out experiments and research alone.  Having  
> reliable
> staff assist you is not unusual either.
>
> Good luck to you,
>
> Tom Jasper
>
>
> Thomas Jasper HT (ASCP) BAS
> Histology Supervisor
> Central Oregon Regional Pathology Services
> Bend, Oregon 97701
> 541/693-2677
> tjasper <@t> copc.net
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Salim
> Yalcin Inan
> Sent: Friday, November 27, 2009 2:44 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] a basic question about immunohistochemistry
>
> Dear All,
>
> Because I am new in immunohistochemistry, I have a basic question  
> about
> it.
> What if your rat dies in the evening or in the weekend, which you are
> doing a chronic experiment and need to collect brain tissue for
> immunohistochemistry? And let's say, the staff did not noticed it to
> inform you on time. Several hours passed since your rat died. There is
> no way to do perfusion. Is it still possible to do  
> immunohistochemistry?
> Thank you very much in advance.
>
> Best regards,
>
> Salim Yalcin Inan, Ph.D.
> (post-doctoral fellow)
> Department of Clinical Neurosciences
> University of Calgary
> syinan <@t> ucalgary.ca
>
>