[Histonet] a basic question about immunohistochemistry

[Thomas Jasper]

Dear Salim,

As you have been informed, doing immunohistochemistry is possible on
this tissue.  After all it's possible to do IHC on any tissue whether
the conditions you want to test under are ideal or not.  

Being chastised on this list and calling your work "bad science" is
totally out of line and certainly does not help you out.  I think some
people would do well to reserve judgment, particularly when there's no
way they can fully understand what's going on with your project.  Having
worked in research myself, I completely understand that animals will
die, at the most inconvenient times, during a study.

First of all you should incorporate the data about the animal dying into
your study notes.  Secondly, there's no harm in running the IHC on this
animal's tissue.  You can use the results comparatively with results
from some perfused tissue later on.

I don't know Salim, some people might call it damage control, or making
the best of a less than ideal situation.  Again, I don't know exactly
what you're working on but it seems there's information worth gathering
despite the circumstances.  I also understand that it's probably next to
impossible to carry out experiments and research alone.  Having reliable
staff assist you is not unusual either.  

Good luck to you,

Tom Jasper


Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/693-2677
tjasper <@t> copc.net 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Salim
Yalcin Inan
Sent: Friday, November 27, 2009 2:44 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] a basic question about immunohistochemistry

Dear All,

Because I am new in immunohistochemistry, I have a basic question about
it.
What if your rat dies in the evening or in the weekend, which you are
doing a chronic experiment and need to collect brain tissue for
immunohistochemistry? And let's say, the staff did not noticed it to
inform you on time. Several hours passed since your rat died. There is
no way to do perfusion. Is it still possible to do immunohistochemistry?
Thank you very much in advance.

Best regards,

Salim Yalcin Inan, Ph.D.
(post-doctoral fellow)
Department of Clinical Neurosciences
University of Calgary
syinan <@t> ucalgary.ca


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet