[Histonet] IBA1-microglia staining on frozen sections
ti fei
tifei <@t> foxmail.com
Thu Nov 26 10:59:11 CST 2009
Very surprising!
I just use PFA fixation after I cut the tissue, and I can stain it again!
Anyone has the experience of staining IBA1 on alcohol/acetone-fixed brain sections before?
In literature it said yes this works.
------------------ Original ------------------
From: "TF"<tifei <@t> foxmail.com>;
Date: Thu, Nov 26, 2009 10:26 PM
To: "Histonet <@t> lists.utsouthwestern.edu"<Histonet <@t> lists.utsouthwestern.edu>;
Subject: [Histonet] IBA1-microglia staining on frozen sections
Hi all, i just can not get this IBA1 (marker of microglia) well stained on my frozen sections fixed with acetone (sections were either dried up in air for 1 hour or fixed immediately). But I always get good staining on PFA-perfused brain tissue.
Will IBA1 antigen reactivity lose in acetone fixation?
2009-11-26
TF
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