[Histonet] IBA1-microglia staining on frozen sections

TF tifei <@t> foxmail.com
Thu Nov 26 08:26:42 CST 2009


Hi all, i just can not get this IBA1 (marker of microglia) well stained on my frozen sections fixed with acetone (sections were either dried up in air for 1 hour or fixed immediately). But I always get good staining on PFA-perfused brain tissue. 

Will IBA1 antigen reactivity lose in acetone fixation?



2009-11-26 



TF 


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