AW: [Histonet] Biopsy fixation

[Gudrun Lang]

Kris,
why don't you leave the skin biopsies in NBF over night? 
At our institute it is usual practice to let this specimens fix until next
day, if they arive after 1 p.m. I cannot see a negative effect.
For me 4 hours in formalin are too less for a good fixation. How long is the
duration of fixation in the infiltration processor? Aren't the biopsies
rather brittle while cutting, due to secondary fixation with ethanol?

Gudrun

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Kalleberg,
Kristopher
Gesendet: Mittwoch, 25. November 2009 00:51
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Biopsy fixation

Hello All,
 
I have a simple fixation question.  Typically when we process 3 mm skin
biopsies we will fix in 10% NBF for 4 hours and then wash in PBS and
then process over night into paraffin and then embed.  If we are unable
to immediately process the 3mm skin biopsies after 4 hr fixation what is
the maximum time we can leave the biopsies in 70% alcohol before
processing into paraffin?  Is there a better reagent, such as PBS, other
than alcohol to leave the biopsy in for a few days before tissue
processing.  Thank you in advance.
 
Kris  
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