AW: [Histonet] Biopsy fixation

Gudrun Lang gu.lang <@t> gmx.at
Wed Nov 25 11:30:27 CST 2009


Kris,
why don't you leave the skin biopsies in NBF over night? 
At our institute it is usual practice to let this specimens fix until next
day, if they arive after 1 p.m. I cannot see a negative effect.
For me 4 hours in formalin are too less for a good fixation. How long is the
duration of fixation in the infiltration processor? Aren't the biopsies
rather brittle while cutting, due to secondary fixation with ethanol?

Gudrun

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Kalleberg,
Kristopher
Gesendet: Mittwoch, 25. November 2009 00:51
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Biopsy fixation

Hello All,
 
I have a simple fixation question.  Typically when we process 3 mm skin
biopsies we will fix in 10% NBF for 4 hours and then wash in PBS and
then process over night into paraffin and then embed.  If we are unable
to immediately process the 3mm skin biopsies after 4 hr fixation what is
the maximum time we can leave the biopsies in 70% alcohol before
processing into paraffin?  Is there a better reagent, such as PBS, other
than alcohol to leave the biopsy in for a few days before tissue
processing.  Thank you in advance.
 
Kris  
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