[Histonet] difference between immunocytochemistry and immunohistochemistry-Fr

[Dong Yujuan]

Hi all, I am using an monoclonal antibody to test a membrane protein (tested appilication include Flow cyt and western blot).
It was succsessfuly used in Immunocytochemistry (cell culture) before. However when I applied it in Immunohistochemistry (Frozen sections, human tissue), there was no signal at all (The fixation is the same). 
Is there so big difference between ICC and IHC-Fr? Is it my problem or the antibody's? 
Many thanks!
 
Celia 
Chinese University of Hong Kong
Hong Kong


      Yahoo!香港提供網上安全攻略，教你如何防範黑客! 請前往 http://hk.promo.yahoo.com/security/ 了解更多!