[Histonet] difference between immunocytochemistry and immunohistochemistry-Fr

Dong Yujuan yujuan_dong <@t> yahoo.com
Tue Nov 24 23:00:40 CST 2009


Hi all, I am using an monoclonal antibody to test a membrane protein (tested appilication include Flow cyt and western blot).
It was succsessfuly used in Immunocytochemistry (cell culture) before. However when I applied it in Immunohistochemistry (Frozen sections, human tissue), there was no signal at all (The fixation is the same). 
Is there so big difference between ICC and IHC-Fr? Is it my problem or the antibody's? 
Many thanks!
 
Celia 
Chinese University of Hong Kong
Hong Kong


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