[Histonet] cryojane tap transfer system

[Merced M Leiker]

Unless I'm missing something, I don't understand why people use this tape? 
It seems like a marketing gimmick to me...ol' fashion' melting of sections 
onto slides works perfectly for us...

?

Regards,
Merced

--On Monday, November 23, 2009 9:01 AM -0500 Richard Pattison 
<richard.pattison <@t> gmail.com> wrote:

> Hi Everybody,
> I was hoping to get some advice - I'm cryosectioning plant tissues and
> transferring sections to slides using the Cryojane system. However, i'm
> having problems in transferring the sections without them falling apart
> during the tape transfer. I'm fixing my tissue for 24 hours in
> ethanol:acetic acid (3:1), embedding in O.C.T, snap-freezing and then
> sectioning at between 2 and 14 microns. The sections seem to be ok but
> whenever i remove the adhesive tape from the slide a large part of the
> tissue is removed with it. As a result I lose the majority of my section.
> I've tried using both 1x and 1/2x slides (CFSA adhesive slides from
> instrumedics) but neither have given satisfactory results.
> Does anyone have any suggestions as to how i could reduce the loss of
> tissue? Any advice would be much appreciated.
> Thanks
> Richard
>
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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

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