[Histonet] cryojane tap transfer system

[Richard Pattison]

Hi Everybody,
I was hoping to get some advice - I'm cryosectioning plant tissues and 
transferring sections to slides using the Cryojane system. However, i'm 
having problems in transferring the sections without them falling apart 
during the tape transfer. I'm fixing my tissue for 24 hours in 
ethanol:acetic acid (3:1), embedding in O.C.T, snap-freezing and then 
sectioning at between 2 and 14 microns. The sections seem to be ok but 
whenever i remove the adhesive tape from the slide a large part of the 
tissue is removed with it. As a result I lose the majority of my 
section. I've tried using both 1x and 1/2x slides (CFSA adhesive slides 
from instrumedics) but neither have given satisfactory results.
Does anyone have any suggestions as to how i could reduce the loss of 
tissue? Any advice would be much appreciated.
Thanks
Richard