[Histonet] RE: Histonet Digest, Vol 72, Issue 20

[Havens, Kimberley]

Concerning Paula Lucas's request for fumes in her lab:

We have a ThermoScientific Hyperclean Fume Extraction Hood that sits on a table-ledge in our Mohs Lab. We have the Hyperclean Hood on the whole time we are processing the tissues. We have not had any problems with fumes. I think the investment of the Hyperclean Fume Hood is worthwhile in keeping the fumes under control. If you need more information concerning the Hyperclean Hood, you can go to www.thermo.com. I hope this answers your question.

Have a great day!~

Kimberley Havens
MOHS Technician


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Thursday, November 19, 2009 12:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 72, Issue 20


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Today's Topics:

   1. Industrial Hygienist/Fumes in lab (Paula Lucas)
   2. Re: Freezing of the  sceletal muscles (Galina Deyneko)
   3. RE: [SPAM-HC] - [Histonet] Industrial Hygienist/Fumes in lab
      -	Email found in subject (Cazares, Ruth)
   4. RE: Re: Freezing of the  sceletal muscles (Morken, Tim)
   5. Re: OSHA shoe requirements for histology (Rene J Buesa)
   6. IHC in Ontario Canada (Gagnon, Eric)
   7. Rat perfusion protocol- what's wrong? (Josephine Garcia)
   8. RE: Histonet Digest, Vol 72, Issue 19 (Joanne Clark)
   9. RE: Fwd: foxp3 ab ihc (Tony Henwood)
  10. RE: OSHA shoe requirements for histology (Ingles Claire )
  11. Sanderson's Rapid Bone Stain and what you can do about it
      (gayle callis)
  12. RE: foxp3 ab ihc (C.M. van der Loos)
  13. Histotech Jobs! (Alisha Taylor)
  14. unscribe (eileen dusek)
  15. Re: unscribe (Roger Moretz)


----------------------------------------------------------------------

Message: 1
Date: Wed, 18 Nov 2009 10:06:41 -0800
From: "Paula Lucas" <plucas <@t> biopath.org>
Subject: [Histonet] Industrial Hygienist/Fumes in lab
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001301ca6879$e167d3d0$0f01a8c0 <@t> biopath.local>
Content-Type: text/plain;	charset="us-ascii"

Hello everyone,

 

I'm hoping someone can help me.  We have a small room in the lab where the
coverslipper and stainer are.  We have the coverslipper attached to a fume
hood which is attached to the ceiling to exhaust the fumes out of the
building.  The stainer has a charcoal filter inside the unit. We have an air
purifier to help with ventilation and we have a fume hood to work under as
well.  With all these precautions, this area still gets a good amount of
fumes lurking around for people to feel uncomfortable with.  We do the badge
monitoring and are always under the limits, but I still would like to get it
better.

 

What kind of ventilation solutions have you done to help minimize the fumes
and/or do you have a contact of a Certified Industrial Hygienist? I would
like one to come and take measurements or monitor the fumes in this area.

 

Thanks in advance,

Paula Lucas

Lab Manager

Bio-Path Medical Group



------------------------------

Message: 2
Date: Wed, 18 Nov 2009 10:13:32 -0800 (PST)
From: Galina Deyneko <galinadeyneko <@t> yahoo.com>
Subject: [Histonet] Re: Freezing of the  sceletal muscles
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <219946.53957.qm <@t> web33103.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Dear Colleagues.
I have couple questions regarding freezing of the mouse / rat muscles.
1. Frida Carson in the book recommends to measure the temperature of the isopentane during the freezing and " do not guess" but I cannot find the thermometer for -150 C. Any information where i can find the thermometer would be helpful.
2. I should freeze the gastronemicus/soleous/plantar muscle complex from rats for muscle size, typing, enzyme staining,  but i really afraid that the specimens would be too big ( even rat gastron. muscle itself is pretty big and thick) and i get a lot of ice crystal artifacts. Could you please share your experience, hints etc, how i should perform my task and receive a good quality specimens.
Thank you in advance.
Galina Dunk,
Novartis, Cambridge MA

---


      

------------------------------

Message: 3
Date: Wed, 18 Nov 2009 12:35:19 -0600
From: "Cazares, Ruth" <RCazares <@t> schosp.org>
Subject: RE: [SPAM-HC] - [Histonet] Industrial Hygienist/Fumes in lab
	-	Email found in subject
To: Paula Lucas <plucas <@t> biopath.org>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<572D1F45B44B3D4096D554B4CB40639C01F26FE996 <@t> EXCHCCRMB.schosp.org>
Content-Type: text/plain; charset="us-ascii"

Hi Paula,

We did the same thing with our coverslipper, venting to the ceiling and out. We also had the stainer vented out through the ceiling and this helped dramatically.  If you do vent your stainer though, you have to remove the charcoal filter, as the filter actually prevents the fumes from being sucked out efficiently.


Ruth Cazares, HT (ASCP)
Histology Supervisor
Department of Pathology
Swedish Covenant Hospital
5145 North California Ave
Chicago, IL 60625

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula Lucas
Sent: Wednesday, November 18, 2009 12:07 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [SPAM-HC] - [Histonet] Industrial Hygienist/Fumes in lab - Email found in subject

Hello everyone,



I'm hoping someone can help me.  We have a small room in the lab where the coverslipper and stainer are.  We have the coverslipper attached to a fume hood which is attached to the ceiling to exhaust the fumes out of the building.  The stainer has a charcoal filter inside the unit. We have an air purifier to help with ventilation and we have a fume hood to work under as well.  With all these precautions, this area still gets a good amount of fumes lurking around for people to feel uncomfortable with.  We do the badge monitoring and are always under the limits, but I still would like to get it better.



What kind of ventilation solutions have you done to help minimize the fumes and/or do you have a contact of a Certified Industrial Hygienist? I would like one to come and take measurements or monitor the fumes in this area.



Thanks in advance,

Paula Lucas

Lab Manager

Bio-Path Medical Group

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Thank you for your cooperation.



------------------------------

Message: 4
Date: Wed, 18 Nov 2009 10:40:59 -0800
From: "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org>
Subject: RE: [Histonet] Re: Freezing of the  sceletal muscles
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<1AAF670737F193429070841C6B2ADD4CF77A850F <@t> EXMBMCB15.ucsfmedicalcenter.org>
	
Content-Type: text/plain; charset=iso-8859-1


Galina, 

Get a FLUKE 51-2 electronic thermometer, then get the thermocouple that detects below -100C. 


Thermocouple Temperature
Measurement accuracy:
Above -100 °C (-148 °F)
J, K, T, E, and N-type** ±[0.05% + 0.3°C (0.5°F) ]
R** and S-type** ±[0.05% + 0.4°C (0.7°F) ] Below -100 °C (-148 °F):
J, K, E, and N-types ±[0.20% + 0.3°C (0.5°F) ] T-type ±[0.50% + 0.3°C (0.5°F) ] Display resolution: 0.1°C / °F / K < 1000° 1°C / °F / K ³ 1000° Measurement range:
J-type: -210°C to 1200°C (-346°F to 2192°F)
K-type: -200°C to 1372°C (-328°F to 2501°F)
T-type: -250°C to 400°C (-418°F to 752°F)
E-type: -150°C to 1000°C (-238°F to 1832°F)
N-type**: -200°C to 1300°C (-328°F to 2372°F)
R** and S-type**: 0°C to 1767°C (32°F to 3212°F)
Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA  
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Galina Deyneko
Sent: Wednesday, November 18, 2009 10:14 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Freezing of the sceletal muscles

Dear Colleagues.
I have couple questions regarding freezing of the mouse / rat muscles.
1. Frida Carson in the book recommends to measure the temperature of the isopentane during the freezing and " do not guess" but I cannot find the thermometer for -150 C. Any information where i can find the thermometer would be helpful.
2. I should freeze the gastronemicus/soleous/plantar muscle complex from rats for muscle size, typing, enzyme staining,  but i really afraid that the specimens would be too big ( even rat gastron. muscle itself is pretty big and thick) and i get a lot of ice crystal artifacts. Could you please share your experience, hints etc, how i should perform my task and receive a good quality specimens.
Thank you in advance.
Galina Dunk,
Novartis, Cambridge MA

---


      
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 5
Date: Wed, 18 Nov 2009 12:21:59 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] OSHA shoe requirements for histology
To: histonet <histonet <@t> lists.utsouthwestern.edu>,	richard wells
	<wa7buxx <@t> yahoo.com>
Message-ID: <414701.30978.qm <@t> web65714.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

That one and also not using open shoes.
René J.

--- On Wed, 11/18/09, richard wells <wa7buxx <@t> yahoo.com> wrote:


From: richard wells <wa7buxx <@t> yahoo.com>
Subject: [Histonet] OSHA shoe requirements for histology
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Date: Wednesday, November 18, 2009, 11:36 AM


It is my understanding that OSHA requires some sort of non-slip shoe to prevent falls due to parafin on floors.  Does anyone have any more information regarding this?
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      

------------------------------

Message: 6
Date: Wed, 18 Nov 2009 15:30:18 -0500
From: "Gagnon, Eric" <gagnone <@t> KGH.KARI.NET>
Subject: [Histonet] IHC in Ontario Canada
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<F93BD6329FC3AE4C8DB116B985FBC31327C3AA94 <@t> KGHMAIL.KGH.ON.CA>
Content-Type: text/plain;	charset="iso-8859-1"

Hi Sheila,
 
What are the Ontario Laboratory Accreditation requirements questions that you have regarding IHC?  We were assessed in spring/09.  We run well over a hundred markers using three Ventana BenchMark XT's.
 
Eric Gagnon MLT
Histology Laboratory 
Kingston General Hospital
Kingston, Ontario, Canada
 
 




------------------------------

Message: 7
Date: Wed, 18 Nov 2009 14:16:28 -0800
From: Josephine Garcia <jbdg <@t> u.washington.edu>
Subject: [Histonet] Rat perfusion protocol- what's wrong?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<ab74448c0911181416j7411464bmef17bc4584631c96 <@t> mail.gmail.com>
Content-Type: text/plain; charset=windows-1252

Hi all,
We are trying to harvest muscle and nerve tissue from Sprague-Dawley rats
(upper leg muscle, sciatic nerve).
Is it necessary to perfuse the animal before harvesting muscle / nerve, or
will simply soaking in 4% PFA do?
Our perfusions often, but not always, fail. By "fail", I mean the rat does
not twitch after Paraformaldehyde is circulated, and the tissue does not get
stiff. Here is our protocol.

·         Inject with 0.5 mL Beuthanasia (pink liquid, draw up with 3mL
syringe) into liver (right abdomen)

·         Open chest cavity on perfusion table by pulling skin on chest up
and cutting away circular region. Pierce diaphragm, cut up through ribs
(towards armpit) with blunt side of scissors down, exposing heart and lungs

·         Inject butterfly needle (connected to pump) into the lobe that
faces the back - go up into the heart from the bottom (see diagram)

·         Turn on perfusion pump (clockwise button) at ~20mL/min

·         Cut (using smallest scissors available) black sac near the heart -
this is the vena cava. If done correctly, the cavity should fill with blood

·         Pump > 60mL of saline from the syringe into the system, draining
the blood. Once the blood being drained turns into a clearer red, switch to
Paraformaldehyde

·         Pump > 150mL of cold 4% Paraformaldehyde. The rat should twitch
and stiffen within minutes if perfusion is successful (twitching occurs ~90%
of the time)


Also, if perfusion is successful, we soak the tissue in 0.4%
paraformaldehyde instead of 4%. Is this correct?


Thanks for the help.
**


------------------------------

Message: 8
Date: Wed, 18 Nov 2009 17:29:58 -0700
From: "Joanne Clark" <jclark <@t> pcnm.com>
Subject: [Histonet] RE: Histonet Digest, Vol 72, Issue 19
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C01142EC9 <@t> mail.pcnm.com>
Content-Type: text/plain;	charset="US-ASCII"


Hi Shiela, I went through an OLA accreditation before I came to work
here the States and it seems to me if you do all the same QC you did for
CAP you will be OK.  You will need to validate your instruments and all
of your IHC protocols and have records to show.  All of your procedures
for all your antibodies will need to be documented and how you QC test
them.  Unless they have changed things, you can use outdated antibodies
with OLA if you have a written procedure that outlines how they are used
and what to do if the QC/QA guidelines are not met.  You will need to
have QC testing records for when a new shipment of antibody is received
from the vendor before being put into use.

Joanne Clark, HT, MLT(CSMLS)
Histology Supervisor
Pathology Consultants of New Mexico
Roswell, NM


Message: 9
Date: Wed, 18 Nov 2009 07:35:11 -0500
From: sheila adey <sheila_adey <@t> hotmail.com>
Subject: [Histonet] IHC  in Ontario Canada
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BAY129-W14A413CD235A011D9CA31F93A30 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"


 

Hello,

 

I've recently started working back in Canada and I am looking to find
other techs that are familiar with the OLA regulations for IHCs. Seems
to be a little different than the CAP guidelines. 



Sheila Adey HT MLT 
 		 	   		  
_________________________________________________________________
Eligible CDN College & University students can upgrade to Windows 7
before Jan 3 for only $39.99. Upgrade now!
http://go.microsoft.com/?linkid=9691819

------------------------------




------------------------------

Message: 9
Date: Thu, 19 Nov 2009 12:01:20 +1100
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] Fwd: foxp3 ab ihc
To: "Marilyn Tyler" <Marilyn.Tyler <@t> uct.ac.za>, "histonet"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B9EAF61856077F47BF9BE2F89AFC5552068537F2 <@t> hedwig.nch.kids>
Content-Type: text/plain; charset="us-ascii"

Marilyn,

We have used Biolegend's Rabbit anti FoxP3 (Cat No 623801) at a 1/20
dilution with citrate retrieval (ER1) on the Bond Max with very good
results.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marilyn
Tyler
Sent: Wednesday, 18 November 2009 6:48 PM
To: histonet
Subject: [Histonet] Fwd: foxp3 ab ihc



Does anybody in histoland use any FOXP3 antibody.  I would like to know
which is the better ab to use for FFPE human material. Posting on behalf
of Heather as she cannot get through to Histonet
>>> Heather McCleod 2009/11/18 08:37 AM >>>
 
 
Thank you
The Histonet ROCKS!!!
Heather
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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------------------------------

Message: 10
Date: Wed, 18 Nov 2009 20:25:08 -0600
From: "Ingles Claire " <CIngles <@t> uwhealth.org>
Subject: RE: [Histonet] OSHA shoe requirements for histology
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<F2F030053F9B7345831BED293A6D57E109A7A3 <@t> UWHC-MAIL01.uwhis.hosp.wisc.edu>
	
Content-Type: text/plain;	charset="iso-8859-1"

No open toed shoes for one.
Claire

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of richard wells
Sent: Wed 11/18/2009 10:36 AM
To: histonet
Subject: [Histonet] OSHA shoe requirements for histology



It is my understanding that OSHA requires some sort of non-slip shoe to prevent falls due to parafin on floors.  Does anyone have any more information regarding this?
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





------------------------------

Message: 11
Date: Wed, 18 Nov 2009 19:25:59 -0700
From: "gayle callis" <gayle.callis <@t> bresnan.net>
Subject: [Histonet] Sanderson's Rapid Bone Stain and what you can do
	about it
To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000401ca68bf$a3360870$e9a21950$@callis <@t> bresnan.net>
Content-Type: text/plain;	charset="us-ascii"

It was good hear that RBS was being picked up and sold by Dorn and Hart, but
you can also make this up yourself in the laboratory.  Cathy
Sanderson/Mayton found a way to make this up easier than the original
formula and Surgipath manufactured it.  

 

The original name was Stevenels Blue and the original  publication for
staining PMMA embedded bone:  

 

Maniatopoulos C et al  (1987)  An improved method for preparing histological
sections of metallic implants.  International J or Oral and Maxillofacial
Implants 1:(1):31 

 

It can be made up in the laboratory although time consuming and messy.  We
devised a way to make it up as quickly as possible.  I will be happy to send
the recipe and preparation method to anyone who needs it privately.  Cathy
did us all a favor by making this up in some proprietary way so we could
purchase it easily.    

 

Gayle M. Callis

HTL/HT/MT(ASCP)

Bozeman MT 59715  



------------------------------

Message: 12
Date: Thu, 19 Nov 2009 08:45:26 +0100
From: "C.M. van der Loos" <c.m.vanderloos <@t> amc.uva.nl>
Subject: [Histonet] RE: foxp3 ab ihc
To: Marilyn.Tyler <@t> uct.ac.za
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <aaf573a73c956a20.4b0505a6 <@t> amc.uva.nl>
Content-Type: text/plain; charset=us-ascii

Marilyn,Clone 236A/E7 (Abcam) worked fine for us. See: De Boer et al. (2007) PlosOne 8:e779 and De Boer et al. (2007) JHC 55:891-898.Cheers, ChrisChris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The NetherlandsDate: Wed, 18 Nov 2009 09:47:49 +0200
From: "Marilyn Tyler" <Marilyn.Tyler <@t> uct.ac.za>
Subject: [Histonet] Fwd: foxp3 ab ihc
To: "histonet" histonet <@t> lists.utsouthwestern.edu

Does anybody in histoland use any FOXP3 antibody.  I would like to know which is the better ab to use for FFPE human material.
Posting on behalf of Heather as she cannot get through to Histonet
>>> Heather McCleod 2009/11/18 08:37 AM >>>
 
Thank you
The Histonet ROCKS!!!
Heather


------------------------------

Message: 13
Date: 19 Nov 2009 09:59:01 -0500
From: Alisha Taylor <alisha <@t> ka-recruiting.com>
Subject: [Histonet] Histotech Jobs!
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1137998437.1258642741372.JavaMail.cfservice <@t> webserver59>
Content-Type: text/plain; charset="utf-8"





Dear Histotechnologists,





 


I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm.  I help place Lab Professionals in permanent positions across the country and I wanted to see if you or someone you know may be interested in exploring other career opportunities?  We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. 

 




I am currently working with a mid-sized nonprofit hospital in southwest Oklahoma.  This area is known for its year round recreational activities as it's located near the mountains and a lake. My client is looking for histotech for their 1st shift position. The ideal candidate will have graduated from an accredited Histotechnology school, will be ASCP certified as an HT or HTL, and will have greater than 1 year of experience in a histology lab. My client offers a very competitive hourly rate, full benefits, and possible relocation assistance, if needed. Please email or call me if you have an interest in learning more about this position.

 










Below is a list of some of the other great opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates.






 

Other Current Histology Opportunities: 

 



Histology Supervisor - Georgia (1st shift)


Histotechnologist- Georgia (1st shift)

Histotechnologist - Southern CA (all shifts)



Histology Supervisor- Southern CA (3rd shift)

Pathology Manager - Bay Area, CA (1st shift)







Histotechnologist - Boston, MA (1st shift)

Histotechnologist - Oklahoma 



Histology Supervisor- Oklahoma (1st shift)



Histotechnologist - New York City, NY (3rd shift) 









Pathology Manager - New York City, NY (1st shift) 


Histotechnologist - Otsego County, NY (Upstate NY)








 





















If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is.  

 

If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future.  I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country.  

 


To view some additional opportunities please visit our website at www.ka-recruiting.com.  
























Sincerely,

 

Alisha R. Taylor, Founder

K.A. Recruiting, Inc.

Your Partner in Healthcare Recruiting

10 Post Office Square 8th Floor SOUTH

Boston, MA 02109

P: (617) 692-2949

F: (617) 507-8009

alisha <@t> ka-recruiting.com

www.ka-recruiting.com

 



------------------------------

Message: 14
Date: Thu, 19 Nov 2009 08:41:23 -0800 (PST)
From: eileen dusek <eileen_dusek <@t> yahoo.com>
Subject: [Histonet] unscribe
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <80487.91368.qm <@t> web50602.mail.re2.yahoo.com>
Content-Type: text/plain; charset=us-ascii




      

------------------------------

Message: 15
Date: Thu, 19 Nov 2009 09:46:38 -0800 (PST)
From: Roger Moretz <stamptrain <@t> yahoo.com>
Subject: Re: [Histonet] unscribe
To: eileen dusek <eileen_dusek <@t> yahoo.com>,
	histonet <@t> lists.utsouthwestern.edu
Message-ID: <798449.19125.qm <@t> web55808.mail.re3.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

First of all, that's not even a word.  Secondly, that's not the way it's done.  I'm sure others will point you in the right direction.

Roger Moretz, Ph.D. (ret.)



----- Original Message ----
From: eileen dusek <eileen_dusek <@t> yahoo.com>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Thu, November 19, 2009 11:41:23 AM
Subject: [Histonet] unscribe




      
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End of Histonet Digest, Vol 72, Issue 20
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Stormont-Vail HealthCare is proud to be in the top 5 percent of hospitals in the country honored by the American Nurses Credentialing Center's Magnet Recognition Program.

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