[Histonet] Re: Freezing of the sceletal muscles

[Morken, Tim]

Galina, 

Get a FLUKE 51-2 electronic thermometer, then get the thermocouple that detects below -100C. 


Thermocouple Temperature
Measurement accuracy:
Above -100 °C (-148 °F)
J, K, T, E, and N-type** ±[0.05% + 0.3°C (0.5°F) ]
R** and S-type** ±[0.05% + 0.4°C (0.7°F) ] Below -100 °C (-148 °F):
J, K, E, and N-types ±[0.20% + 0.3°C (0.5°F) ] T-type ±[0.50% + 0.3°C (0.5°F) ] Display resolution: 0.1°C / °F / K < 1000° 1°C / °F / K ³ 1000° Measurement range:
J-type: -210°C to 1200°C (-346°F to 2192°F)
K-type: -200°C to 1372°C (-328°F to 2501°F)
T-type: -250°C to 400°C (-418°F to 752°F)
E-type: -150°C to 1000°C (-238°F to 1832°F)
N-type**: -200°C to 1300°C (-328°F to 2372°F)
R** and S-type**: 0°C to 1767°C (32°F to 3212°F)
Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA  
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Galina Deyneko
Sent: Wednesday, November 18, 2009 10:14 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Freezing of the sceletal muscles

Dear Colleagues.
I have couple questions regarding freezing of the mouse / rat muscles.
1. Frida Carson in the book recommends to measure the temperature of the isopentane during the freezing and " do not guess" but I cannot find the thermometer for -150 C. Any information where i can find the thermometer would be helpful.
2. I should freeze the gastronemicus/soleous/plantar muscle complex from rats for muscle size, typing, enzyme staining,  but i really afraid that the specimens would be too big ( even rat gastron. muscle itself is pretty big and thick) and i get a lot of ice crystal artifacts. Could you please share your experience, hints etc, how i should perform my task and receive a good quality specimens.
Thank you in advance.
Galina Dunk,
Novartis, Cambridge MA

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