[Histonet] B5 alternate

[Drew Meyer]

Essentially it's a zinc formalin.

Drew

Sent from my iPhone

On Nov 16, 2009, at 4:53 PM, John Kiernan <jkiernan <@t> uwo.ca> wrote:

> What is this "B plus" fixative?  Is it a trade-secret brew?
>
> John Kiernan
> Anatomy, UWO
> London, Canada
> = = =
> ----- Original Message -----
> From: Anne van Binsbergen <annigyg <@t> gmail.com>
> Date: Friday, November 13, 2009 10:01
> Subject: Re: [Histonet] B5 alternate
> To: Drew Meyer <41dmb41 <@t> gmail.com>
> Cc: "Knutson, Deanne" <DKnutson <@t> primecare.org>, "histonet <@t> lists.utsouthwestern.edu 
> " <histonet <@t> lists.utsouthwestern.edu>
>
>> B plus is the one - we use it as stated and it works like a charm
>> immunos are good too - we are proudly mercury free
>> Annie
>>
>> 2009/11/13 Drew Meyer <41dmb41 <@t> gmail.com>
>>
>>> In my lab, we're using B-Plus Fixative by BBC
>> Biochemical.  I know
>>> many other labs that are also using it as their B5
>> alternative.  The
>>> biggest pro, of course, is that it's mercury free and not hazardous.
>>> Also, it's not as sensitive to over-fixation like B5 is.  The
>>> guideline I use is 4 hours minimum fixation for Bone Marrows and
>>> Lymphoid tissue.  After that, routine formalin processing
>> is all you
>>> need to do.  You can, however, leave the specimen in B-
>> Plus for up to
>>> 48 hours without adversely effecting the tissue sample.
>>>
>>> Hope this help.
>>>
>>> Drew Meyer
>>>
>>> On Fri, Nov 13, 2009 at 09:43, Knutson, Deanne
>> <DKnutson <@t> primecare.org>> wrote:
>>>> Am looking for advice on what alternate fixative to use to
>> replace B5.
>>> What
>>>> do the majority of you histonetters use?  Does it work
>> well with immunos?
>>>> The pros and cons?  Thank you for your recommendations.
>>>>
>>>>
>>>>
>>>> Deanne Knutson
>>>>
>>>> Anatomic Pathology Supervisor
>>>>
>>>> St. Alexius Medical Center
>>>>
>>>> 900 E. Broadway
>>>>
>>>> Bismarck, North Dakota  58506
>>>>
>>>> (701)-530-6730
>>>>
>>>> dknutson <@t> primecare.org <mailto:dknutson <@t> primecare.org>
>>>>
>>>>
>>>>
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>>>
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>>
>>
>>
>> -- 
>> Anne van Binsbergen (Hope)
>> Abu Dhabi
>> UAE
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