[Histonet] B5 alternate

John Kiernan jkiernan <@t> uwo.ca
Mon Nov 16 15:53:24 CST 2009


What is this "B plus" fixative?  Is it a trade-secret brew?  
 
John Kiernan
Anatomy, UWO
London, Canada
= = = 
----- Original Message -----
From: Anne van Binsbergen <annigyg <@t> gmail.com>
Date: Friday, November 13, 2009 10:01
Subject: Re: [Histonet] B5 alternate
To: Drew Meyer <41dmb41 <@t> gmail.com>
Cc: "Knutson, Deanne" <DKnutson <@t> primecare.org>, "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>

> B plus is the one - we use it as stated and it works like a charm
> immunos are good too - we are proudly mercury free
> Annie
> 
> 2009/11/13 Drew Meyer <41dmb41 <@t> gmail.com>
> 
> > In my lab, we're using B-Plus Fixative by BBC 
> Biochemical.  I know
> > many other labs that are also using it as their B5 
> alternative.  The
> > biggest pro, of course, is that it's mercury free and not hazardous.
> > Also, it's not as sensitive to over-fixation like B5 is.  The
> > guideline I use is 4 hours minimum fixation for Bone Marrows and
> > Lymphoid tissue.  After that, routine formalin processing 
> is all you
> > need to do.  You can, however, leave the specimen in B-
> Plus for up to
> > 48 hours without adversely effecting the tissue sample.
> >
> > Hope this help.
> >
> > Drew Meyer
> >
> > On Fri, Nov 13, 2009 at 09:43, Knutson, Deanne 
> <DKnutson <@t> primecare.org>> wrote:
> > > Am looking for advice on what alternate fixative to use to 
> replace B5.
> >  What
> > > do the majority of you histonetters use?  Does it work 
> well with immunos?
> > > The pros and cons?  Thank you for your recommendations.
> > >
> > >
> > >
> > > Deanne Knutson
> > >
> > > Anatomic Pathology Supervisor
> > >
> > > St. Alexius Medical Center
> > >
> > > 900 E. Broadway
> > >
> > > Bismarck, North Dakota  58506
> > >
> > > (701)-530-6730
> > >
> > > dknutson <@t> primecare.org <mailto:dknutson <@t> primecare.org>
> > >
> > >
> > >
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> 
> 
> 
> -- 
> Anne van Binsbergen (Hope)
> Abu Dhabi
> UAE
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