[Histonet] amnion tissue

[Monfils, Paul]

I fold the membrane back and forth on a wet piece of lens paper in a petrie dish. This gives me an elongate mass several layers thick and about 1.5 cm in width.  I wrap this in lens paper and insert it into a nylon biopsy bag for processing. This holds the whole thing flat. For embedding I unwrap the specimen (which now adheres together pretty well), slice the whole thing right down the center with a scalpel blade, and embed both halves with the cut edge down, right next to each other in the same block. This provides a lot of material to look at microscopically, a dozen or more full length cross sections of the membrane.