[Histonet] Re: B5 alternate

[Robert Richmond]

I loved B5 fixative, and before that Zenker's, but they're gone and
they're not coming back.

In my personal expereience in a number of pathology services, the
various muchly-touted B5 substitutes offer very little morphologic
advantage over neutral buffered formalin.

Unless it's fixed pretty early in the morning, tissue needs to sit
overnight in neutral buffered formalin before being decalcified or
processed. Pathologists and their clinicians are eventually going to
have to learn to live with this delay.

Bob Richmond
Samurai Pathologist
Knoxville TN