[Histonet] RE: Histonet Digest, Vol 72, Issue 14
Valantou Grover
vgrover <@t> polysciences.com
Fri Nov 13 12:24:17 CST 2009
Oh beyond fun! We'll have more fun to come. Did you make it obvious?
Valantou Grover, HT/HTL(ASCP), PA, MBA
Biosciences Product Line Manager
Polysciences, Inc.
400 Valley Road
Warrington, PA 18976
Fax: 1-800-343-3291
Phone number: 1-800-523-2575 X7418
Direct:1-215-488-7418
Cell phone: 1-215-409-8327
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: None
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 72, Issue 14
Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-owner <@t> lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
1. Re: B5 alternate (Drew Meyer)
2. Re: B5 alternate (Anne van Binsbergen)
3. Re: Muscle injection site in rats (Merced M Leiker)
4. RE: B5 alternate (Morin, Mary Anne)
5. Re: B5 alternate (Rene J Buesa)
6. "Email Spoofed." addresses were highjacked (Akemi Allison-Tacha)
7. gp350 (Steffi Linnerbauer)
8. Re: "Email Spoofed." addresses were highjacked (Peter Carroll)
9. Staining Spurrs (Scott Hooten)
10. RE: SPAM-LOW: [Histonet] RE: Looking for VEGF and FGF-2
antibodies (Patsy Ruegg)
----------------------------------------------------------------------
Message: 1
Date: Fri, 13 Nov 2009 09:53:11 -0500
From: Drew Meyer <41dmb41 <@t> gmail.com>
Subject: Re: [Histonet] B5 alternate
To: "Knutson, Deanne" <DKnutson <@t> primecare.org>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<ebcbe38a0911130653n39774495uac3c4372b2c16a6b <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
In my lab, we're using B-Plus Fixative by BBC Biochemical. I know
many other labs that are also using it as their B5 alternative. The
biggest pro, of course, is that it's mercury free and not hazardous.
Also, it's not as sensitive to over-fixation like B5 is. The
guideline I use is 4 hours minimum fixation for Bone Marrows and
Lymphoid tissue. After that, routine formalin processing is all you
need to do. You can, however, leave the specimen in B-Plus for up to
48 hours without adversely effecting the tissue sample.
Hope this help.
Drew Meyer
On Fri, Nov 13, 2009 at 09:43, Knutson, Deanne <DKnutson <@t> primecare.org>
wrote:
> Am looking for advice on what alternate fixative to use to replace B5.
What
> do the majority of you histonetters use? Does it work well with immunos?
> The pros and cons? Thank you for your recommendations.
>
>
>
> Deanne Knutson
>
> Anatomic Pathology Supervisor
>
> St. Alexius Medical Center
>
> 900 E. Broadway
>
> Bismarck, North Dakota 58506
>
> (701)-530-6730
>
> dknutson <@t> primecare.org <mailto:dknutson <@t> primecare.org>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 2
Date: Fri, 13 Nov 2009 19:00:23 +0400
From: Anne van Binsbergen <annigyg <@t> gmail.com>
Subject: Re: [Histonet] B5 alternate
To: Drew Meyer <41dmb41 <@t> gmail.com>
Cc: "Knutson, Deanne" <DKnutson <@t> primecare.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<f8332fbe0911130700x69097984w78cdc785817c80bb <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
B plus is the one - we use it as stated and it works like a charm
immunos are good too - we are proudly mercury free
Annie
2009/11/13 Drew Meyer <41dmb41 <@t> gmail.com>
> In my lab, we're using B-Plus Fixative by BBC Biochemical. I know
> many other labs that are also using it as their B5 alternative. The
> biggest pro, of course, is that it's mercury free and not hazardous.
> Also, it's not as sensitive to over-fixation like B5 is. The
> guideline I use is 4 hours minimum fixation for Bone Marrows and
> Lymphoid tissue. After that, routine formalin processing is all you
> need to do. You can, however, leave the specimen in B-Plus for up to
> 48 hours without adversely effecting the tissue sample.
>
> Hope this help.
>
> Drew Meyer
>
> On Fri, Nov 13, 2009 at 09:43, Knutson, Deanne <DKnutson <@t> primecare.org>
> wrote:
> > Am looking for advice on what alternate fixative to use to replace B5.
> What
> > do the majority of you histonetters use? Does it work well with
immunos?
> > The pros and cons? Thank you for your recommendations.
> >
> >
> >
> > Deanne Knutson
> >
> > Anatomic Pathology Supervisor
> >
> > St. Alexius Medical Center
> >
> > 900 E. Broadway
> >
> > Bismarck, North Dakota 58506
> >
> > (701)-530-6730
> >
> > dknutson <@t> primecare.org <mailto:dknutson <@t> primecare.org>
> >
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
------------------------------
Message: 3
Date: Fri, 13 Nov 2009 10:06:20 -0500
From: Merced M Leiker <leiker <@t> buffalo.edu>
Subject: Re: [Histonet] Muscle injection site in rats
To: "Wilson, Carol" <Carol.Wilson <@t> ricerca.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <77681A9F79D8824DB2E0EBB7 <@t> CDYwxp1931.ad.med.buffalo.edu>
Content-Type: text/plain; charset=us-ascii; format=flowed
We've had this problem before! The best we could do was to shave the area
we wanted to inject, then used an ink tattooing device our animal
facilities had which basically dipped a needle into a permanent nontoxic
bright green dye that you then punch the skin with right where you want to
inject. Do it the day before you inject, so the excess dye has time to come
off. The next day you see the little dye mark in the puncture site and
that's your bulls' eye for where you do your injection. It will stay for
weeks at least. Seemed to work for our purposes!
We did it with hamsters and it was a 2 person job: one to hold the animal
while punching/injecting, the other to pull the leg straight by the foot.
Or the one to hold the animal with the other holding the leg and
punching/injecting.
(Sometimes we tried using a Sharpie to circle the site on the shaved skin
so we could locate it easier weeks down the road, but then you have to
re-circle it every day because it wears of quickly with their grooming and
etc.)
Maybe there are other, better methods out there...it would be great to hear
them!!
Regards,
Merced
--On Friday, November 13, 2009 7:40 AM -0500 "Wilson, Carol"
<Carol.Wilson <@t> ricerca.com> wrote:
> Hi All,
>
> Is there a way to isolate the point of injection for histology on an
> intramuscular injection into a rat thigh? Any suggestions or protocols
> would be appreciated.
>
> Thanks,
>
> Carol
>
>
>
> Carol Wilson, HT(ASCP)
>
> Lead Technician/Histology
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)
No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.
------------------------------
Message: 4
Date: Fri, 13 Nov 2009 10:14:13 -0500
From: "Morin, Mary Anne" <MMorin <@t> Lifespan.org>
Subject: RE: [Histonet] B5 alternate
To: "Knutson, Deanne" <DKnutson <@t> primecare.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<130E8991F210424096EFC6F42EA33B2463955F <@t> LSCOEXCH1.lsmaster.lifespan.org>
Content-Type: text/plain; charset="iso-8859-1"
Hi, we replaced B5 with B-Plus Fixative, we use it for our bone marrow bx's
and aspirates. They seem to be happy with the
results, many IHC's are ordered on the bone marrows< Haven't heard any
complaints. I order it from BBC Biochemical
Catalog # 1751 for 1 gallon phone # is 1-800-635-4477.
Have A Good One!!
Marie Anne Morin H.T. ASCP
Path Tech Specialist
Histology Lab
Rhode Island Hospital
Office 444-7196
Pager 350-7384
Fax 444-8514
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Knutson,
Deanne
Sent: Friday, November 13, 2009 9:43 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] B5 alternate
Am looking for advice on what alternate fixative to use to replace B5. What
do the majority of you histonetters use? Does it work well with immunos?
The pros and cons? Thank you for your recommendations.
Deanne Knutson
Anatomic Pathology Supervisor
St. Alexius Medical Center
900 E. Broadway
Bismarck, North Dakota 58506
(701)-530-6730
dknutson <@t> primecare.org <mailto:dknutson <@t> primecare.org>
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Fri, 13 Nov 2009 07:16:32 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] B5 alternate
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>, DeanneKnutson
<DKnutson <@t> primecare.org>
Message-ID: <921384.74040.qm <@t> web65712.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
B5 is an inheritance of the "toxic good old days". It is really a good
fixative, but after thorough testing, my hematologist (a very talented but
picky pathologist) agreed that the ubiquitous yethumble Neutral Buffered
Formalin produced equivalent results.
There is one caveat though: the pH HAS to be EXACTLY 7 and the washings to
be done also with phosphate buffer at pH7.
Other than than you can eliminate the mercury.
Reni J.
--- On Fri, 11/13/09, Knutson, Deanne <DKnutson <@t> primecare.org> wrote:
From: Knutson, Deanne <DKnutson <@t> primecare.org>
Subject: [Histonet] B5 alternate
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Date: Friday, November 13, 2009, 9:43 AM
Am looking for advice on what alternate fixative to use to replace B5. What
do the majority of you histonetters use? Does it work well with immunos?
The pros and cons? Thank you for your recommendations.
Deanne Knutson
Anatomic Pathology Supervisor
St. Alexius Medical Center
900 E. Broadway
Bismarck, North Dakota 58506
(701)-530-6730
dknutson <@t> primecare.org <mailto:dknutson <@t> primecare.org>
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Fri, 13 Nov 2009 07:33:33 -0800 (PST)
From: Akemi Allison-Tacha <akemiat3377 <@t> yahoo.com>
Subject: [Histonet] "Email Spoofed." addresses were highjacked
To: histo net <histonet <@t> pathology.swmed.edu>
Message-ID: <529402.12738.qm <@t> web113818.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi All,
The email below was sent to me in response to the Scam. I guess someone
from histonet contacted him regarding the scam, which was spammed. It's
interesting to find out how vulnerable we all are. Yahoo's customer support
has had high volumes of similar problems.
Akemi,
Boy! Peter needs an Oxycontin! Maybe more than
one.
Don't worry about this. The correct term is being "Email Spoofed." From my
friends, it happens when some computer hijacker either creates content on a
new or existing web site that you ended up clicking on. Peter suggests it
is "Where" you visited, but it could be anywhere, even on a legitimate site.
Some people I've talked to think Microsoft's web page is loaded with "Spoof"
and "Spyware!"
Sometimes, it is a computer worm or virus affecting your computer. Most of
the time, it is associated with your email
provider. [Most of my friends with Yahoo email have been "Spoofed."]
I would run a up-to-date anti-virus software, and (especially if that
doesn't help) ask for help from Yahoo to quarantine or fix your address.
You probably have it fixed already. If
not, it will do it again until someone stops it. It IS only email. So
what???
Hugh-Hawaii
------------------------------
Message: 7
Date: Fri, 13 Nov 2009 16:46:12 +0100
From: "Steffi Linnerbauer" <S_T_E_F_F_I <@t> gmx.net>
Subject: [Histonet] gp350
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20091113154612.308820 <@t> gmx.net>
Content-Type: text/plain; charset="iso-8859-1"
Dear all,
I try to establish an EBV gp350 staining on human EBV positive tumors but
don4t get a result until now. I use a hybridoma supernatant as antibody and
don4t get a result in my positive control.
Is there anyone who have already experience with gp350 antibodies on FFPF or
cryo tissues?
What are good antibodies? any special tipps for the staining?
thanks in advance
--
Jetzt kostenlos herunterladen: Internet Explorer 8 und Mozilla Firefox 3.5 -
sicherer, schneller und einfacher! http://portal.gmx.net/de/go/chbrowser
------------------------------
Message: 8
Date: Fri, 13 Nov 2009 10:52:21 -0500
From: Peter Carroll <carrolpb <@t> umdnj.edu>
Subject: Re: [Histonet] "Email Spoofed." addresses were highjacked
To: Akemi Allison-Tacha <akemiat3377 <@t> yahoo.com>
Cc: histo net <histonet <@t> pathology.swmed.edu>
Message-ID: <4AFD80B5.1070506 <@t> umdnj.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
> Boy! Peter needs an Oxycontin! Maybe more than one.
Since I've already apparently made the impression of being up tight, I
may as well go on and also add that, on top of the ~15 off-topic emails
we've already gotten concerning an email scam you fell for, I sort of
feel that sending someone else's privately-mailed insult of a perfect
stranger to an entire mailing list to be a bit, well, tactless.
Oh well, at least its friday, eh? :)
Akemi Allison-Tacha wrote:
> Hi All,
>
> The email below was sent to me in response to the Scam. I guess someone
from histonet contacted him regarding the scam, which was spammed. It's
interesting to find out how vulnerable we all are. Yahoo's customer support
has had high volumes of similar problems.
>
> Akemi,
>
> Boy! Peter needs an Oxycontin! Maybe more than
> one.
>
> Don't worry about this. The correct term is being "Email Spoofed." From
my friends, it happens when some computer hijacker either creates content on
a new or existing web site that you ended up clicking on. Peter suggests it
is "Where" you visited, but it could be anywhere, even on a legitimate site.
Some people I've talked to think Microsoft's web page is loaded with "Spoof"
and "Spyware!"
>
> Sometimes, it is a computer worm or virus affecting your computer. Most
of the time, it is associated with your email
> provider. [Most of my friends with Yahoo email have been "Spoofed."]
>
> I would run a up-to-date anti-virus software, and (especially if that
doesn't help) ask for help from Yahoo to quarantine or fix your address.
>
> You probably have it fixed already. If
> not, it will do it again until someone stops it. It IS only email. So
what???
>
> Hugh-Hawaii
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
------------------------------
Message: 9
Date: Fri, 13 Nov 2009 11:11:27 -0500
From: "Scott Hooten" <Hooten <@t> medinst.com>
Subject: [Histonet] Staining Spurrs
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4AFD3EDF.8FD5.00E5.0 <@t> medinst.com>
Content-Type: text/plain; charset=US-ASCII
Hello All,
I recently began working with Spurr's Resin and was working on doing an H&E
stain. The H&E stain I use for my MMA does not have the best results on
Spurr's and was wondering if anyone has an H&E staining technique for
Spurr's that they could share with me. Any help is greatly appreciated.
Thanks.
Scott R. Hooten
Histology Technician
MED Institute
1 Geddes Way
West Lafayette, IN 47906
765-464-0817 ext. 1115
------------------------------
Message: 10
Date: Fri, 13 Nov 2009 09:20:23 -0700
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: SPAM-LOW: [Histonet] RE: Looking for VEGF and FGF-2
antibodies
To: "'C.M. van der Loos'" <c.m.vanderloos <@t> amc.uva.nl>,
<histonet <@t> lists.utsouthwestern.edu>
Cc: PMonfils <@t> Lifespan.org
Message-ID: <3269D2B1748C43AAA7AC8383F79B2F8C <@t> Patsyoffice>
Content-Type: text/plain; charset="us-ascii"
Check out www.peprotech.com for these antibodies.
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of C.M. van der
Loos
Sent: Friday, November 13, 2009 4:19 AM
To: histonet <@t> lists.utsouthwestern.edu
Cc: PMonfils <@t> Lifespan.org
Subject: SPAM-LOW: [Histonet] RE: Looking for VEGF and FGF-2 antibodies
Hi Paul,We just completed a study into different VEGF-A antibodies. Our
paper is excepted for J Histochem Cytochem (www.jhc.org) and is available as
PDF under exPRESS at September 28th. Thermo/Labvision RB-9031 came out as
best antibody.Cheers,Chris Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands Date: Thu, 12 Nov 2009 10:26:48 -0500
From: "Monfils, Paul" <PMonfils <@t> Lifespan.org>
Subject: [Histonet] Looking for VEGF and FGF-2 antibodies
To: <histonet <@t> lists.utsouthwestern.edu>
I am having a difficult time finding two antibodies I need. Two companies I
ordered them from have discontinued them.
VEGF and FGF-2
The tissue is human. Monoclonals preferred but at this point I'll use
polyclonals if necessary.
Does anyone know a vendor who has them?
Thanks.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 72, Issue 14
****************************************
More information about the Histonet
mailing list