[Histonet] B5 alternate

[Rene J Buesa]

B5 is an inheritance of the "toxic good old days". It is really a good fixative, but after thorough testing, my hematologist (a very talented but picky pathologist) agreed that the ubiquitous yethumble Neutral Buffered Formalin produced equivalent results. 
There is one caveat though: the pH HAS to be EXACTLY 7 and the washings to be done also with phosphate buffer at pH7.
Other than than you can eliminate the mercury.
René J.

--- On Fri, 11/13/09, Knutson, Deanne <DKnutson <@t> primecare.org> wrote:


From: Knutson, Deanne <DKnutson <@t> primecare.org>
Subject: [Histonet] B5 alternate
To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu>
Date: Friday, November 13, 2009, 9:43 AM


Am looking for advice on what alternate fixative to use to replace B5.  What
do the majority of you histonetters use?  Does it work well with immunos?
The pros and cons?  Thank you for your recommendations.



Deanne Knutson

Anatomic Pathology Supervisor

St. Alexius Medical Center

900 E. Broadway

Bismarck, North Dakota  58506

(701)-530-6730

dknutson <@t> primecare.org <mailto:dknutson <@t> primecare.org> 



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