[Histonet] B5 alternate

[Anne van Binsbergen]

B plus is the one - we use it as stated and it works like a charm
immunos are good too - we are proudly mercury free
Annie

2009/11/13 Drew Meyer <41dmb41 <@t> gmail.com>

> In my lab, we're using B-Plus Fixative by BBC Biochemical.  I know
> many other labs that are also using it as their B5 alternative.  The
> biggest pro, of course, is that it's mercury free and not hazardous.
> Also, it's not as sensitive to over-fixation like B5 is.  The
> guideline I use is 4 hours minimum fixation for Bone Marrows and
> Lymphoid tissue.  After that, routine formalin processing is all you
> need to do.  You can, however, leave the specimen in B-Plus for up to
> 48 hours without adversely effecting the tissue sample.
>
> Hope this help.
>
> Drew Meyer
>
> On Fri, Nov 13, 2009 at 09:43, Knutson, Deanne <DKnutson <@t> primecare.org>
> wrote:
> > Am looking for advice on what alternate fixative to use to replace B5.
>  What
> > do the majority of you histonetters use?  Does it work well with immunos?
> > The pros and cons?  Thank you for your recommendations.
> >
> >
> >
> > Deanne Knutson
> >
> > Anatomic Pathology Supervisor
> >
> > St. Alexius Medical Center
> >
> > 900 E. Broadway
> >
> > Bismarck, North Dakota  58506
> >
> > (701)-530-6730
> >
> > dknutson <@t> primecare.org <mailto:dknutson <@t> primecare.org>
> >
> >
> >
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-- 
Anne van Binsbergen (Hope)
Abu Dhabi
UAE