[Histonet] B5 alternate

[Drew Meyer]

In my lab, we're using B-Plus Fixative by BBC Biochemical.  I know
many other labs that are also using it as their B5 alternative.  The
biggest pro, of course, is that it's mercury free and not hazardous.
Also, it's not as sensitive to over-fixation like B5 is.  The
guideline I use is 4 hours minimum fixation for Bone Marrows and
Lymphoid tissue.  After that, routine formalin processing is all you
need to do.  You can, however, leave the specimen in B-Plus for up to
48 hours without adversely effecting the tissue sample.

Hope this help.

Drew Meyer

On Fri, Nov 13, 2009 at 09:43, Knutson, Deanne <DKnutson <@t> primecare.org> wrote:
> Am looking for advice on what alternate fixative to use to replace B5.  What
> do the majority of you histonetters use?  Does it work well with immunos?
> The pros and cons?  Thank you for your recommendations.
>
>
>
> Deanne Knutson
>
> Anatomic Pathology Supervisor
>
> St. Alexius Medical Center
>
> 900 E. Broadway
>
> Bismarck, North Dakota  58506
>
> (701)-530-6730
>
> dknutson <@t> primecare.org <mailto:dknutson <@t> primecare.org>
>
>
>
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