[Histonet] Re: CD31

[Melanie Black]

Hi Cindy

We found that the coating for the slides interfered with staining,  
and it took many hours to work that out!! If you have good staining  
using coated slides, no problem. Why not try one of each?

Regards
Melanie

On 12 Nov 2009, at 6:06 PM, Baranowski Cynthia wrote:

> Melanie,
> I saw your posting on Histonet in response to CD31 on zinc fixed  
> rat tissue.  You stated that the sections must be mounted onto  
> uncoated slides.  Why is that necessary?
> I will be attempting to work up this antibody on zinc-fixed rat  
> tissue embedded in methyl methacrylate.
> Regards,
> Cindy Baranowski
> Senior Histologist
> Plastic Histology
>
> SJTRI
> 380-B Northyards Blvd.
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Melanie Black
082 469 3352

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