[Histonet] Tissue falling off slide

[Rene J Buesa]

I think that the tissues have been too long in the alcohol. I would suggest hydrate the tissues and process them again with less time in the  alcohols.
René J.

--- On Thu, 11/12/09, John Shelley <jshelley <@t> burnham.org> wrote:


From: John Shelley <jshelley <@t> burnham.org>
Subject: [Histonet] Tissue falling off slide
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Thursday, November 12, 2009, 3:49 PM


Hi Histonetters,

This is a second request for help. I know that this is a pretty rudimentary question but I need help. I have is group that has given me 175 mouse heart specimens for processing and embedding and returned the specimens for them to  start microtoming them and the tissue is falling off the slide. They brought some blocks back for me to cut and I placed the sections on three types of slides Superfrost Plus, Colorfrost Plus which are basically the same and Polysine slide and still had falling off but not at the degree of how their sections fell off. I was thinking that it could be a water issue in the water bath or a tissue processing problem that may be causing it. I process with isopropanol- 6 stations( 30 minutes each) all 100%-and I placed in xylene- 1 station 40 minutes and three paraffins 45 minutes each @60 degrees. The tissue looked perfect when they came out of the processor and I embedded them. Any help and advice would be appreciated.  Thanks!!!

John


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