[Histonet] IHC on N-cadherin, CD146, CD144,ELF97 TRAcP.

[Adam .]

I use rabbit anti-human N-Cadherin (clone YS from IBL), and it stains mouse
bone sections and also mouse cells by Western blot. In my hands, it works
great on unretrieved sections fixed with 10% zinc buffered formalin fixed
sections (but not neutral buffered formalin or paraformaldehyde fixed
sections even with antigen retrieval). I don't have it directly conjugated,
but with indirect immunofluorescence, the cells are quite positive so you
may be able to directly conjugate it yourself and get a decent signal.

Adam

On Thu, Nov 5, 2009 at 9:38 AM, julia hough <julia.m.hough <@t> hotmail.co.uk>wrote:

>
>
>
> Dear histonetters.
>
>
>
> I am trying to perform several new IHC stains on mouse
> tibia. However, I am having trouble finding literature on some of them. I
> would
> like antibodies that work on mouse tissue/bone that are preferably
> conjugated
> to a fluorchrome.
>
>
>
> If anyone has any potentially protocols or information where
> I could get primary antibodies unconjugated/conjugated for the following
> that
> would be lovely:
>
>
>
> N-cadherin, CD146, CD144,ELF97 TRAcP.
>
>
>
> Thanks for your time.
>
>
>
> Julia
>
>
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