[Histonet] RE: trichrome stain on GMA bone sections

[Patsy Ruegg]

I have a trichrome for GMA as well, it uses green instead of aniline blue
and some other modifications.
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of gayle callis
Sent: Saturday, May 30, 2009 9:01 AM
To: 'Histonet'
Subject: [Histonet] RE: trichrome stain on GMA bone sections

Ricki, 

 

I have a Modified Oserhoff (sp) Massons Trichrome method for bone sections
in GMA but it requires a really thin sections (1 um) and no microwave
heating.  Bob Skinner had great success with this modified staining method.
There  is also a publication in J of Histotechnology on this protocol, many
years back.    I can dig  the method  out of my pre-computer day files for
you if you wish.  

 

Doing trichromes on GMA embedded tissues is tricky due to the nature of dyes
(aniline blue) and the dye molecular weights.  Often the GMA stains blue
too??  

 

Gayle M. Callis 

HTL(ASCP)HT,MT

Bozeman MT 

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