[Histonet] Multispecimen tissue blocks /sausage

[Anne van Binsbergen]

i am very interested in your technique - please do share here on the
histonet if possible
thanks
Annie in Abu Dhabi

2009/5/29 O'Donnell, Bill <billodonnell <@t> catholichealth.net>

>
> Carmen,
>
> I have developed a VERY inexpensive methodology for making tissue
> micro-array blocks for immuno controls. It is a bit time consuming, but no
> more than making sausage blocks. I would be happy to share it with you if
> you are interested. It conserves precious tissue and comfortably places 16+
> pieces of tissue on a slide in about the space of a nickel, utilizing
> materials you very likely have in your lab.
>
> William (Bill) O'Donnell, HT (ASCP) QIHC
> Lead Histologist
> Good Samaritan Hospital
> 10 East 31st Street
> Kearney, NE 68847
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of carmen loiselle
> Sent: Thursday, May 28, 2009 2:23 PM
> To: pathology education
> Subject: [Histonet] Multispecimen tissue blocks /sausage
>
>
> To all histonetters,
>
>
>
> I need your expertise for the preparation of sausage multispecimen block
> for quality control in immunoshistochemistry lab.  At first my pathologist
> wanted to try microarray but because of the cost he had decided to go for
> sausage instead.  I would greatly appreciate any information regarding this
> matter starting with the procurement of the specimen, thickness for cutting
> (ideal, do you use a punch like for skin biopsy)  and preparing the sausage
> (with or without wrapping , I've read that some people are using amnionic
> membrane), etc.  Do you retrieve the specimen wanted directly from the
> paraffin block then gathering all the specimen needed into the same cassette
> , etc.
>
>
>
> As you can see I'm trying to get all the pertinent information before
> starting this project which will be very challenging into our institution.
>
>
>
> Thank you in advance for all the info you'll share
>
>
>
> Have a nice day !
>
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-- 
Anne van Binsbergen (Hope)
Abu Dhabi
UAE