[Histonet] Advice on fatty liver sectioning

[Andrea Grantham]

-18˚ is the temp I use when I section liver for ORO and I usually cut  
the sections at 5 microns. It sounds like your tissue was not frozen  
correctly.

Andi



Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cell Biology and Anatomy
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algranth <@t> email.arizona.edu
Tel: 520.626.4415     Fax: 520.626.2097

"happy slicing and dicing and may all your stains work perfectly" -  
Paula Sicurello
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On May 28, 2009, at 12:20 PM, Kinsley, David wrote:

> Hi,
> I have some very fatty mouse livers that I have to obtain frozen  
> sections for Oil red O and IHC.  I have tried sectioning from -18 to  
> -30 degrees and from 5um to 10um and the liver just seems to  
> compress, pull away from the OCT, or roll up on itself while the OCT  
> sections well.  When I section below -30 parts of the liver have  
> chatter in them as if they are too cold.  Does anyone have any  
> suggestions on what the best cutting temperature would be for these?
>
> I have successfully cut normal and less fatty livers at 5um ad 10 um  
> at -18 and -25 degrees respectively, it is these extremely fatty  
> livers that are giving me problems.  Any advice is appreciated.
>
> Dave
>
>
>
>
>
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