[Histonet] Hypoxia in Post-Mortem Samples

[Patten, Nicole (NIH/NIAAA) [F]]

Hey there!

I have some post-mortem brain samples (FFPE) with varying PMI's and I'd like to determine the hypoxia levels (and location) at time of death (to identify if this may play a role in the disease I am studying). First of all, does anyone have any suggestions as to a good hypoxia antibody? Second of all, does anyone know how the PMI would affect the staining for hypoxia?

Thanks, I'd appreciate any comments!

Nicole J. Patten
Post-Baccalaureate Fellow/IRTA
National Institutes of Health