[Histonet] IHC control debate

[Tony Henwood]

Here is one reference that might be of use:

Philippe Bertheau "Variability of immunohistochemical reactivity on
stored paraffin slides" J Clin Pathol 1998;51:370-374

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lynette
Pavelich
Sent: Wednesday, 27 May 2009 4:16 AM
To: histonet <@t> lists.utsouthwestern.edu; fudo <@t> ufl.edu
Subject: [Histonet] IHC control debate


Hello,
I need help from all of our IHC expert guru's!  We have a debate going
on about the known positive control blocks.  All are FFPE with 8-48 hrs
in formalin.  All have been tested and have been approved for use as
positive controls.  We currently cut the positive control from the block
as needed. I have learned that some controls do start to deteriorate
over time, tonsils for example.  But, is there a list, an article,
SOMETHING that can guide me so that we can cut some of these controls
ahead of time? 
This would not only save tech time, but would further the use of our
controls.  In cutting the block, some of the good control is wasted and
some positives are hard to find. My argument is that the purchased
controls work wonderfully, even after a very long time and our improved
detection kits have improved so much over the years, it makes this
possible.

If there is no published article out there, I implore you experts to
please help us in this area.  Would also like to hear from the technical
experts from our suppliers too!  Until then, the debate continues
on!!!!!!!

Thank you!!
Lynette

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