[Histonet] autoflurescence of cytospin cells

[FU,DONGTAO]

Hi, all

  I need you help.I was doing immunoflurescent staining on 
cytospin mouse tumor cells and I noticed high autoflurescence 
background of the cells, it happened even before I added first 
antibody. I tried 4% paraformaldehyde or acetone fixation, but 
either of them gave me the same result.

  Does anyone here have experiences on decreasing the 
autoflurescence on the cytospin cells? Or any suggestions? The PI 
wants to see double staining, so it is impossible to do chromagen 
methods.

  Thank you,


Ann Dongtao Fu
University of Flodrida