[Histonet] cloudy formalin in vip tissue processor

[Kemlo Rogerson]

hehehe, thanks...
 
Only got it completely the wrong way around didn't I? Freida is correct,
I think!
 

Kemlo Rogerson  

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work.                    

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________________________________

From: Freida Carson [mailto:freidac <@t> sbcglobal.net] 
Sent: 26 May 2009 13:38
To: Kemlo Rogerson
Subject: RE: [Histonet] cloudy formalin in vip tissue processor


I'm sorry, but formalin is an additive, non-coagulant fixative.  I am
not sending this through the histonet, but did want to correct you.  I
imagine others will also.  Formalin adds on at the amino group.
 
Freida Carson

--- On Tue, 5/26/09, Kemlo Rogerson <Kemlo.Rogerson <@t> waht.swest.nhs.uk>
wrote:


	
	From: Kemlo Rogerson <Kemlo.Rogerson <@t> waht.swest.nhs.uk>
	Subject: RE: [Histonet] cloudy formalin in vip tissue processor
	To: "Christine I. Braaten" <CBraaten <@t> Cheshire-Med.COM>,
histonet <@t> lists.utsouthwestern.edu
	Date: Tuesday, May 26, 2009, 6:16 AM
	
	
	That's a 'harsh' fixative; used it years and years ago as it
mordants
	the haematoxylin. I think that I still stand by the harsh
effects of
	that fixative on coagulating protein and that the culprit is
precisely
	that; coagulated protein from using a additive fixative (as you
know
	formalin is a coagulant, non-additive fixative, normally).
	
	
	
	Kemlo Rogerson    
	e-mail kemlorogerson <@t> nhs.net
<http://us.mc825.mail.yahoo.com/mc/compose?to=kemlorogerson@nhs.net>  if
not at work.            
	DD   01934 647057 or extension 3311     Mob 07749 754194; 
	Embrace uncertainty. Hard problems rarely have easy solutions.
--Jonah
	Lehrer
	This e-mail is confidential and privileged. If you are not the
intended
	recipient please accept my apologies; please do not disclose,
copy or
	distribute information in this e-mail or take any action in
reliance on
	its contents: to do so is strictly prohibited and may be
unlawful.
	Please inform me that this message has gone astray before
deleting it.
	Thank you for your co-operation
	
	
	
	-----Original Message-----
	From: histonet-bounces <@t> lists.utsouthwestern.edu
<http://us.mc825.mail.yahoo.com/mc/compose?to=histonet-bounces@lists.uts
outhwestern.edu> 
	[mailto:histonet-bounces <@t> lists.utsouthwestern.edu
<http://us.mc825.mail.yahoo.com/mc/compose?to=histonet-bounces@lists.uts
outhwestern.edu> ] On Behalf Of
	Christine I. Braaten
	Sent: 22 May 2009 17:36
	To: histonet <@t> lists.utsouthwestern.edu
<http://us.mc825.mail.yahoo.com/mc/compose?to=histonet@lists.utsouthwest
ern.edu> 
	Subject: [Histonet] cloudy formalin in vip tissue processor
	
	Thanks to all who have replied to my query. I probably should
have
	mentioned that we use zinc formalin in our processor and as our
primary
	fixative. I have spoken to both Sakura and Anatech (supplier of
zinc
	formalin) and feel more confused. Anatech says it's a change in
pH that
	causes the zinc to precipitate and that xylene will cause a
whole lot
	more problems than a little cloudiness. Sakura thinks the zinc
formalin
	is the problem. We clean the retort with the cleaning program
every
	morning and make sure to dry the retort so I'm not sure how
xylene would
	get into the formalin. If this brings up any other issues I
would
	appreciate more feedback. Thanks 
	
	
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