[Histonet] antigen retrieval-pressure cooker-CD44- CD24-canine tissue

[anjan kumar]

hello everyone,
                     I wanted to know the exact stepwise protocol for antigen retrieval using a pressure cooker. i tried using some protocol but there is no staining. Kindly tell me the detailed protocol, like when i should place the slides into the pressure cooker and from when i should count the time. 

And i am using mouse monoclonals CD44 & CD24 labvision in canine tissue, has anyone got experience with these antibodies as citrate buffer doesnt at all seems to work.
plz send me some information on this.

Regards,
Dr. Anjan Kumar.K.R

M.V.Sc Scholar

Dept. of Veterinary Pathology

Madras Veterinary College

Chennai-7

India

email: drvet_anjan <@t> hotmail.com

Phone: +91-9940475801



_________________________________________________________________
Live Search extreme As India feels the heat of poll season, get all the info you need on the MSN News Aggregator
http://news.in.msn.com/National/indiaelections2009/aggregator/default.aspx