[Histonet] cloudy formalin in vip tissue processor

Jennifer MacDonald JMacDonald <@t> mtsac.edu
Fri May 22 15:52:03 CDT 2009


Christine,
Is the zinc formalin on the tissue processor or is the tissue fixed in 
zinc formalin and then put on the tissue processor with regular 10%NBF in 
the stations?
Jennifer




"Christine I. Braaten" <CBraaten <@t> Cheshire-Med.COM> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
05/22/2009 09:39 AM

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[Histonet] cloudy formalin in vip tissue processor






Thanks to all who have replied to my query. I probably should have
mentioned that we use zinc formalin in our processor and as our primary
fixative. I have spoken to both Sakura and Anatech (supplier of zinc
formalin) and feel more confused. Anatech says it's a change in pH that
causes the zinc to precipitate and that xylene will cause a whole lot
more problems than a little cloudiness. Sakura thinks the zinc formalin
is the problem. We clean the retort with the cleaning program every
morning and make sure to dry the retort so I'm not sure how xylene would
get into the formalin. If this brings up any other issues I would
appreciate more feedback. Thanks 


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