[Histonet] decal solution

Rene J Buesa rjbuesa <@t> yahoo.com
Fri May 22 15:05:13 CDT 2009


1- with nitric acid at high concentration as is used to decalcify, pH will be below 1
2- you cannot "overdecalcify" a tissue, but you can affect its staining characteristics after a harsh decalcification.
René J.

--- On Fri, 5/22/09, Jennifer Campbell <jcampbell <@t> vdxpathology.com> wrote:


From: Jennifer Campbell <jcampbell <@t> vdxpathology.com>
Subject: [Histonet] decal solution
To: histonet <@t> lists.utsouthwestern.edu
Date: Friday, May 22, 2009, 1:14 PM


Does anyone know what the optimal pH for a nitric acid decal solution
should be?  Right now our's is reading below 1.0 when tested and we are
having problems with our tissue being overdecalcified.  Thank you in
advance.

Jennifer C.
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