[Histonet] RE: Whole mount processing

[Shirley A. Powell]

Hi Allison, 

I just responded to a similar request.  I can help you.  I do whole mount sections, macros, and will be presenting a workshop at NSH (#40) in Birmingham AL in October.  Whole prostates were done at the hospital where I used to work and we were able to section them on the regular rotary Leica microtomes and mounting on 2x2 slides.  If larger they were sectioned on a sliding microtome and mounted on 3.25" x 4" slides.  For larger organs, brain, heart, lung, I use 5" x 7" slides.  Give me a call at 478-301-2374.  

Shirley Powell

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Thursday, May 21, 2009 11:38 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Wholemount processing

Hello to all in histoland.  My lab is looking to start processing whole prostate specimens and doing the wholemount
process.  Can anyone give me a starting point on how to proceed.  I would need information on processing times,
embedding and cutting equipment, staining information and the glass that you mount the specimens on.  Any help in this matter would be greatly appreciated.
 
Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
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