[Histonet] Antibodies for Alport syndrome

[Young Kwun]

Hi All,

Has anyone had experience doing IHC on FFPE tissues with Collagen Type IV
subunit antibodies?

These are Collagen Type IV alpha 1, alpha 3 and alpha 5 chains.  They are
used for the diagnosis of Alport syndrome and all antibodies are from KAMIYA
BIOMEDICAL COMPANY, Seatle. At present, it seems that only COL4A5 is working
on FFPE tissues.

I tried with (or without) various pre-treatment before the primary
incubation with the dilution of 1:50 to 1:500.

I am using BondMax autostainer and I also tried Decloaking chamber HIER with
manual staining.

I was wondering whether there would be other source for these antibodies.

Thank you. 

 

Regards,

 

Young

 

 

 

Young Kwun

Senior Hospital Scientist

Dept. of Anatomical Pathology

Concord Hospital 

Concord NSW 2139 Australia

Tel)61-2-9767-6075

Fax)61-2-9767-8427

kwuny <@t> email.cs.nsw.gov.au