[Histonet] fite for nocardia
Madary, Joseph
MadaryJ <@t> MedImmune.com
Wed May 20 12:51:15 CDT 2009
I recall not using just xylene but a mixture of xylene and peanut oil even for the spins the oil is required to coat the bacteria and since there is no paraffin the xylene still acts as kind of a diluent for the peanut oil. Also do not forget ocardia filaments need to be differentiated with sulfuric acid not hydrochloric for best results. At AFIP we called it a Coates Fite after an Air Force guy named Jerry Coates.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, May 20, 2009 1:07 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 66, Issue 23
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Today's Topics:
1. Re Nikon Diaphot 300 (Norman, Michael)
2. IHC certification (Achstetter, Virginia A.)
3. Career Opportunity in Chicago area (JimR0712 <@t> comcast.net)
4. Looking for career opportunity in Chicago area
(JimR0712 <@t> comcast.net)
5. Trying to post (af46 <@t> buffalo.edu)
6. CLIA regulations (Jonathan De La Rosa)
7. PathNet HLA Module (Brown, Lori)
8. PO/Zylene on cytospins for Fites (Carrie Disbrow)
9. Hi Tony, (Carrie Disbrow)
10. Thanks to those who helped find Larry M. plus (Leroy Brown)
11. Cryoprotection Issues with Unfixed Mouse Hearts (Adam Bazama)
12. RE: Cryoprotection Issues with Unfixed Mouse Hearts (gayle callis)
13. RE: IHC cert. (Lee & Peggy Wenk)
14. Sucrose infiltration and Bone question... (Jamie E Erickson)
15. Histotechnician 2 opportunity in Portland, OR
(Fletcher, Annette M)
16. Job Opening In Sacramento, CA (Jennifer McAnally)
17. microwave antigen retrieval (Kimberly Tuttle)
18. (Tissue Processor's) Used, Refurbished or Lease Agreement
(Akemi Allison-Tacha)
19. Re: microwave antigen retrieval (rjbuesa <@t> yahoo.com)
----------------------------------------------------------------------
Message: 1
Date: Mon, 18 May 2009 17:00:17 -0400
From: "Norman, Michael" <cmnorman <@t> aacc.edu>
Subject: [Histonet] Re Nikon Diaphot 300
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<3614638D4C701B438F335F0EFA8F6EBC68C6271112 <@t> AACC-MAILBOX.aacc.cc.md.us>
Content-Type: text/plain; charset="us-ascii"
Does anyone have a .pdf copy of the instructions manual for the Nikon Diaphot 300 Inverted Microscope they could send me?
Thanks,
Michael Norman
________________________________
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------------------------------
Message: 2
Date: Tue, 19 May 2009 07:31:58 -0400
From: "Achstetter, Virginia A." <Virginia.Achstetter <@t> us.army.mil>
Subject: [Histonet] IHC certification
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<127D612DBCD77546A12C4746F7491C7F053C1AD6 <@t> lewis.afip.osd.mil>
Content-Type: text/plain; charset="us-ascii"
How do I go about finding the study material needed for the IHC exam,
how do I prepare, etc.
Thanks
Ginny Achstetter
------------------------------
Message: 3
Date: Tue, 19 May 2009 17:14:08 +0000 (UTC)
From: JimR0712 <@t> comcast.net
Subject: [Histonet] Career Opportunity in Chicago area
To: Histonet2 <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<387056283.11337581242753248724.JavaMail.root <@t> sz0098a.emeryville.ca.mail.comcast.net>
Content-Type: text/plain; charset=utf-8
Last week, my former employer, Loyola University Medical Center in Maywood, Il., layed off 440 employees (about 8 % of the workforce).
I was the Manager of Anatomic Pathology (Histo/Cyto/Autopsy/Transcription/MohsLabs/Clerical Support) and now am looking for a career opportunity in the Chicago area.
Please let me know if you hear of any career opportunities.
Thanks.
------------------------------
Message: 4
Date: Tue, 19 May 2009 17:47:43 +0000 (UTC)
From: JimR0712 <@t> comcast.net
Subject: [Histonet] Looking for career opportunity in Chicago area
To: Histonet2 <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1482254971.11355411242755263324.JavaMail.root <@t> sz0098a.emeryville.ca.mail.comcast.net>
Content-Type: text/plain; charset=utf-8
I am looking for a career opportunity.
----- Forwarded Message -----
From: JimR0712 <@t> comcast.net
To: "Histonet2" <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, May 19, 2009 12:14:08 PM GMT -06:00 US/Canada Central
Subject: Career Opportunity in Chicago area
Last week, my former employer, Loyola University Medical Center in Maywood, Il., layed off 440 employees (about 8 % of the workforce).
I was the Manager of Anatomic Pathology (Histo/Cyto/Autopsy/Transcription/MohsLabs/Clerical Support) and now am looking for a career opportunity in the Chicago area.
Please let me know if you hear of any career opportunities.
Thanks.
------------------------------
Message: 5
Date: Tue, 19 May 2009 14:39:52 -0400
From: af46 <@t> buffalo.edu
Subject: [Histonet] Trying to post
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <14581.1242758392 <@t> buffalo.edu>
Content-Type: text/plain; charset="utf-8"
just testing to see if I can post
------------------------------
Message: 6
Date: Tue, 19 May 2009 12:45:13 -0700 (PDT)
From: Jonathan De La Rosa <jdhisto <@t> yahoo.com>
Subject: [Histonet] CLIA regulations
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <638245.37316.qm <@t> web110701.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello Histoneters,
I was wondering if someone could contact me offline about CLIA regulations and/or inspections. I have a few concerns. Any help would be appreciated.
Thanks in advance.
JD
jdhisto <@t> yahoo.com
------------------------------
Message: 7
Date: Tue, 19 May 2009 16:08:33 -0400
From: "Brown, Lori" <LBrown <@t> getvitalized.com>
Subject: [Histonet] PathNet HLA Module
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F3E05D9A9D1F834A84D8E52143FA59CE2E2DBC6AD3 <@t> vcsmail.getvitalized.com>
Content-Type: text/plain; charset="us-ascii"
I'm hoping to network with some wonderful Pathologists (and informaticists). I'm wondering if anyone out there has ever used or been a part of an implementation of Cerner Millennium's PathNet HLA module for tissue transplant. Any networking and advice is greatly appreciated!!
Lori M. Brown, SPHR, CIR
Recruiting Manager
Vitalize Consulting Solutions | 410-392-5332 o | 484-356-3781 c | 484-734-6168 f | www.getvitalized.com<http://www.getvitalized.com/>
________________________________
Erase your problems. See how in booth #530 at the International MUSE May 25th - 29th.
________________________________
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------------------------------
Message: 8
Date: Tue, 19 May 2009 17:19:36 -0400
From: "Carrie Disbrow" <disbrc <@t> shands.ufl.edu>
Subject: [Histonet] PO/Zylene on cytospins for Fites
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4A12EA2D.72AC.0059.0 <@t> shands.ufl.edu>
Content-Type: text/plain; charset=US-ASCII
Hi Rene,
Thanks for the info. We are doing a AFB Fites for Nocardia. There is filamentous bacteria in the patient GMS and the docs are differentiating the type. I did not deparaffinize the patient cytospin slides and I did deparaffinize the patient control with Z/PO. The problem I had been anticipating was that the patient and control did not have the same procedure. I was thinking of it as a CAP issue,,,, but then realized we don't have the same preparation for any stains when using cytospins and controls, ie GMS, Mucin, irons, all have controls that need deparaffinization. DUH!
Anyway the stain did not work. It looks like the Methylene blue is too heavy and blocking the carbol fuschin. We use Dako's Artisan stainer, which I love and are having to rework this stain.
Thanks again,
Carrie
------------------------------
Message: 9
Date: Tue, 19 May 2009 17:22:45 -0400
From: "Carrie Disbrow" <disbrc <@t> shands.ufl.edu>
Subject: [Histonet] Hi Tony,
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4A12EAEA.72AC.0059.0 <@t> shands.ufl.edu>
Content-Type: text/plain; charset=US-ASCII
Hi Tony,
Thanks for the info. I understand the discussion topic. I just wish their was one straight answer! But then this is histology!
Carrie
------------------------------
Message: 10
Date: Tue, 19 May 2009 14:33:22 -0700
From: Leroy Brown <rhbrown1 <@t> histocs.com>
Subject: [Histonet] Thanks to those who helped find Larry M. plus
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<mailman.0.1242838800.2068.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain; charset="iso-8859-1";
I was able to speak to Larry M. about my tissue processor problems. thank you all for your help.
I am now looking for perhaps a broken MVP-1 or 2 since I am no longer able to get the CRT display parts to fix mine. they are interchangeable and if I could find a discarded MVP I could get mine running again. Anyone have a mothballed one sitting in the basement?
Thanks
LeRoy Brown HT(ASCP)HTL
www.histocs.com
Everson, Wa 98247
------------------------------
Message: 11
Date: Tue, 19 May 2009 16:44:08 -0500
From: "Adam Bazama" <baza0013 <@t> umn.edu>
Subject: [Histonet] Cryoprotection Issues with Unfixed Mouse Hearts
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001c9d8ca$f0d14610$d273d230$@edu>
Content-Type: text/plain; charset="us-ascii"
Does anyone have any suggestions or protocols for cryoprotecting unfixed
mouse heart tissue? Some of the ones I have been cryosectioning lately have
been damaged, I think because of improper cryoprotection.
Thanks!
Adam
baza0013 <@t> umn.edu
------------------------------
Message: 12
Date: Tue, 19 May 2009 16:49:36 -0600
From: "gayle callis" <gayle.callis <@t> bresnan.net>
Subject: RE: [Histonet] Cryoprotection Issues with Unfixed Mouse
Hearts
To: "'Adam Bazama'" <baza0013 <@t> umn.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002101c9d8d4$17177750$454665f0$@callis <@t> bresnan.net>
Content-Type: text/plain; charset="us-ascii"
Adam,
It is not a good to sucrose cryoprotect fresh tissues. Only well fixed
tissues should be cryoprotected to lessen freezing artifact caused by large
water ice crystals. Charles Scouten, on his website discusses this, and if
one cryoprotects fresh tissues, you change the osmolarity within
tissues/cells and the tissues shrivel up. This is probably the
damage/changes you are observing. Tissues that are well fixed with NBF or a
PFA mixture should be sucrose cryoprotected.
For fresh tissues, dissect heart from mouse, embed and snap freeze with a
proper RAPIC snap freezing method. If you would like, I will privately send
a simple snap freezing that eliminates use of precooled isopentane. Avoid
freezing in a cryostat or a freezer, even -80C as freezing will be too slow
and at warmer temperture than true/rapidsnap freezing methods. This will
also cause freezing artifact in both fresh or sucrose cryoprotected fixed
tissues.
Be sure to read Dr. Scouten's comments on snap freezing and its effects on
tissues - it is very informative.
Gayle Callis
HTL(ASCP)HT,MT
Bozeman MT
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Adam Bazama
Sent: Tuesday, May 19, 2009 3:44 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cryoprotection Issues with Unfixed Mouse Hearts
Does anyone have any suggestions or protocols for cryoprotecting unfixed
mouse heart tissue? Some of the ones I have been cryosectioning lately have
been damaged, I think because of improper cryoprotection.
Thanks!
Adam
baza0013 <@t> umn.edu
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 13
Date: Tue, 19 May 2009 20:19:46 -0400
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: RE: [Histonet] IHC cert.
To: "'Ginny .'" <gachstetter <@t> yahoo.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <CF81428E7D1C4A2BA07525CD51623D5B <@t> HPPav2>
Content-Type: text/plain; charset="iso-8859-1"
For all information about certification exams (e.g., HT, HTL, SLS
(specialist in laboratory safety), or on qualification exams (e.g., QIHC),
go to
www.ascp.org
Click on Laboratory Professional
Click on Certification
Click on either "Get Certified" or "Get Qualified"
Under Get Qualified, there is information on study material (#5)
- Reading list
- Topic outline
Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ginny .
Sent: Tuesday, May 19, 2009 7:37 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC cert.
Where can I find the study material needed to prepare for the IHC exam?
When on the ASCP website but found nothing.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Wed, 20 May 2009 09:32:09 -0400
From: Jamie E Erickson <jamie.erickson <@t> abbott.com>
Subject: [Histonet] Sucrose infiltration and Bone question...
To: "histonet histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<OFD9B767D9.0023C368-ON852575BC.0045C681-852575BC.004A5B1A <@t> abbott.com>
Content-Type: text/plain; charset="US-ASCII"
Hi All,
I have two question about sucrose infiltration....First, I
have infiltrated many mouse brain with sucrose 30% in the past and it has
always been at 4degrees C (dc) and I'm asking myself now why? My mouse
brain are fixed in paraformaldehyde or formaldehyde for 24 hours prior so
why sucrose @ 4dc? Would it not infiltrate better (faster) at room temp?
I could not find out why 4dc...
My real problem is that I am tiring to get good mouse tibia/femur frozen
sections and I am using D-profile blade and a tape transfer method. It
worked great on my rats sections but not mouse so I think the sucrose is
not getting in to the bone. I can't use the sinking tissue as an
indicator because the bone sinks right away.
Should I go longer in sucrose (maybe 48 Hrs) but at 4dc or room temp ?
Would adding 2 % DSMO to sucrose facilitate penetration..??
Any suggestions would be great..
Thanks have a great day..
Jamie
_______________________________
Jamie Erickson
Sr. Research Associate II M.S. HTL (ASCP)
Department: DSMP
Abbott Bioresearch Center
100 Research Drive
Worcester, MA 01605-4341
508-688-3134
FAX: 508-793-4895
e-mail: jamie.erickson <@t> abbott.com
------------------------------
Message: 15
Date: Tue, 19 May 2009 11:23:22 -0700
From: "Fletcher, Annette M" <Annette.Fletcher <@t> providence.org>
Subject: [Histonet] Histotechnician 2 opportunity in Portland, OR
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<284596E350CB0743BB500B18475E7A0B022A6562 <@t> wn1223.or.providence.org>
Content-Type: text/plain; charset="iso-8859-1"
Good Afternoon Histonetters,
Annette Fletcher here with Providence Health and Services in Portland, Oregon. We are seeking a Histotech for our Regional Lab in Portland, OR. This is an opportunity to work for a visionary Manager who is dedicated to growing and developing her Histo staff. We have a state of the art lab, highly competitive compensation and an excellent benefit plan. We also offer relocation assistance.
You can email me at annette.fletcher <@t> providence.org with your interest.
Thank you,
Annette M Fletcher
Senior Recruiter
Providence Health & Services, Employment
1235 NE 47th Avenue - Suite 200
Portland, OR 97213
T: 503-215-5840
F: 503-215-5770
Annette.fletcher <@t> providence.org
Confidentiality notice: The information contained in this email message including attachments is confidential and is intended only for the use of the individual or entity named above and others who have been specifically authorized to receive it. If you are not the intended recipient, you are hereby notified that any use, unauthorized dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please delete immediately or if any problems occur with transmission, please notify me immediately by telephone at 503-215-5840 or by reply e-mail at annette.fletcher <@t> providence.org. Thank you!
DISCLAIMER:
This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message.
------------------------------
Message: 16
Date: Wed, 20 May 2009 09:32:11 -0500
From: "Jennifer McAnally" <j_mcanally <@t> hotmail.com>
Subject: [Histonet] Job Opening In Sacramento, CA
To: <Histonet <@t> lists.utsouthwestern.edu>,
"'LMargraf <@t> childmed.dallas.tx.us.'"
Message-ID: <BAY128-DAV10A6BF775779467F928864E3580 <@t> phx.gbl>
Content-Type: text/plain; charset="us-ascii"
**
*Hello Histonetters,*
**
*I have an opening for a Lead/Supervisory Histotechnologist in Sacramento,
CA. *
**
* Please review the job description below.
If you know someone qualified for this position, please forward this
information. For anyone interested, please forward your resume to
jennifer <@t> phcconsulting.com for consideration.
This Lab is part of a multi-lab company. This particular Lab processes
around 300 cases a day. This person will be accountable for the direct
supervision and operation of the lab and staff.
We need someone who can support the company's growth oriented business plan
and has an entrepreneurial spirit. This person will be vital to the overall
success of the lab so the following characteristics are necessary: Great
leadership skills, Ability to train others, Able to smoothly manage the
workflow of the lab, Great attitude, energetic and pleasant personality.
3-5 years experience as a Histotechnologist, with at least 1-2 years in
leadership role preferred.
Salary range is $70k to $95k depending on experience. Performance bonuses,
full benefits, healthcare, health savings accounts, 401k and accrued
vacation. Relo package offered for the right person.
Jennifer McAnally
PHC Consulting
888-263-5688 ext 300
------------------------------
Message: 17
Date: Wed, 20 May 2009 11:26:32 -0400
From: "Kimberly Tuttle" <ktuttle <@t> umm.edu>
Subject: [Histonet] microwave antigen retrieval
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4A13E8E7.90CE.001A.3 <@t> umm.edu>
Content-Type: text/plain; charset=US-ASCII
Can someone post instructions for microwave antigen retrieval... my pressure cooker broke and I need an immediate solution.
Kimberly C. Tuttle HT (ASCP)
Pathology Biorepository and Research Core
University of Maryland
Room NBW58, UMMC
22 S. Greene St
Baltimore, MD 21201
(410) 328-5524
(410) 328-5508 fax
This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail.
------------------------------
Message: 18
Date: Wed, 20 May 2009 08:34:20 -0700 (PDT)
From: Akemi Allison-Tacha <akemiat3377 <@t> yahoo.com>
Subject: [Histonet] (Tissue Processor's) Used, Refurbished or Lease
Agreement
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <780411.19431.qm <@t> web31301.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Good Morning out there in Histo Land!
Calling all Vendors. I have recently accepted a new position as the Histology Manager at APMG Labs in Los Gatos, CA. We are down one Tissue Processor. The Executive Administrator thought we would be purchasing a used VIP from one of our affiliates, but unfortunately this possibility has fallen through.
I would appreciate any Vendors who have Tissue Processor's for sale that are Used or Refurbished. I am also open to the possibility of negotiating lease agreements. I realize warranties and service agreements are extremely important.
Please e-mail me any available options.
Thanks,Akemi
Akemi Allison-Tacha BS, HT (ASCP) HTL
Histology Manager
Associated Pathology Medical Group Laboratories
105A Cooper Ct. Los Gatos, CA 95032
W: (408)884.2718
W: E-Mail: aallison-tacha <@t> apmglab.com
P: E-Mail: akemiat3377 <@t> yahoo.com
------------------------------
Message: 19
Date: Wed, 20 May 2009 09:05:18 -0700 (PDT)
From: rjbuesa <@t> yahoo.com
Subject: Re: [Histonet] microwave antigen retrieval
To: histonet <histonet <@t> lists.utsouthwestern.edu>, Kimberly Tuttle
<ktuttle <@t> umm.edu>
Message-ID: <848945.6183.qm <@t> web65710.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
You use the MW to heat the HIER solution and the slides in it. Once the buffer boiled with the slides, remove the container with the slides and place it in either a water bath or a steamer at 98ºC during 20 minutes. After that place the container over the counter during another 20 minutes.
Wash with PBS and start the staining procedure (either manual or automated).
René J.
--- On Wed, 5/20/09, Kimberly Tuttle <ktuttle <@t> umm.edu> wrote:
From: Kimberly Tuttle <ktuttle <@t> umm.edu>
Subject: [Histonet] microwave antigen retrieval
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Date: Wednesday, May 20, 2009, 11:26 AM
Can someone post instructions for microwave antigen retrieval... my pressure cooker broke and I need an immediate solution.
Kimberly C. Tuttle HT (ASCP)
Pathology Biorepository and Research Core
University of Maryland
Room NBW58, UMMC
22 S. Greene St
Baltimore, MD 21201
(410) 328-5524
(410) 328-5508 fax
This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail.
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------------------------------
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End of Histonet Digest, Vol 66, Issue 23
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