[Histonet] AFB Fites of cytospins

[Tony Henwood]

If they are cytospins they will not need dewaxing so no vegetable oil.
But having said this there is a school of thought that suggests that the
use of vegetable oil may aid in the identification of Fite positive
organisms (by ?coating the bugs).

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Carrie
Disbrow
Sent: Tuesday, 19 May 2009 8:13 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] AFB Fites of cytospins


Hello Histonet.
Has anyone had experience and good results doing AFB Fites on cytospin
slides from BAL's? Do you use the peanut oil/xylene (PO/Z) solution or
just 100% peanut oil? Or does the cytospin slide need any peanut oil?
After the deparaffinization step, if it can be called that, we are going
to stain using a Dako Artisan special stainer. One problem I foresee is
that the control will be de-arffinized in the traditional PO/Z while the
patient slide may not. Also, I was told that Cytolyte contains alcohol
and asked that the slides not be prepared using Cytolyte. Thanks for
your comments. Please respond back to the histonet if possible and not
my email address. 
Carrie
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