[Histonet] AFB Fites of cytospins

Carrie Disbrow disbrc <@t> shands.ufl.edu
Mon May 18 17:12:34 CDT 2009


Hello Histonet.
Has anyone had experience and good results doing AFB Fites on cytospin slides from BAL's?
Do you use the peanut oil/xylene (PO/Z) solution or just 100% peanut oil? Or does the cytospin slide need any peanut oil?
After the deparaffinization step, if it can be called that, we are going to stain using a Dako Artisan special stainer. One problem I foresee is that the control will be de-arffinized in the traditional PO/Z while the patient slide may not.
Also, I was told that Cytolyte contains alcohol and asked that the slides not be prepared using Cytolyte.
Thanks for your comments. Please respond back to the histonet if possible and not my email address. 
Carrie


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